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glycophorin b, High-throughput genotyping assays for identification of glycophorin B deletion variants in population studies

High-throughput genotyping assays for identification of glycophorin B deletion variants in population studies

November 16, 2021

A nexus of miR-1271, PAX4 and ALK/RYK influences the cytoskeletal architectures in Alzheimer’s Disease and Type 2 Diabetes

November 16, 2021
slco1a1, Time Course of Renal Transcriptomics after Subchronic Exposure to Ochratoxin A in Fisher Rats
Ria Kits

Time Course of Renal Transcriptomics after Subchronic Exposure to Ochratoxin A in Fisher Rats

November 16, 2021
magnetic beads antibody, Analytical methods of antibody surface coverage and orientation on bio-functionalized magnetic beads: application to immunocapture of TNF-α
Ria Kits

Analytical methods of antibody surface coverage and orientation on bio-functionalized magnetic beads: application to immunocapture...

November 16, 2021
complement fixation testing, Brucella suis Seroprevalence and Associated Risk Factors in Dogs in Eastern Australia, 2016 to 2019
Ria Kits

Brucella suis Seroprevalence and Associated Risk Factors in Dogs in Eastern Australia, 2016 to 2019

November 16, 2021
igg1k, A Retrospective Study to Assess the Efficacy, Safety and Drug Survival of Secukinumab in Plaque Psoriasis Patients in China
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A Retrospective Study to Assess the Efficacy, Safety and Drug Survival of Secukinumab in Plaque...

November 16, 2021
basal salt, Understanding the electrochemistry of "water-in-salt" electrolytes: basal plane highly ordered pyrolytic graphite as a model system
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Understanding the electrochemistry of “water-in-salt” electrolytes: basal plane highly ordered pyrolytic graphite as a model...

November 16, 2021
qpcr covid, SARS-CoV-2 UK, South African and Brazilian Variants in Karachi- Pakistan
Ria Kits

SARS-CoV-2 UK, South African and Brazilian Variants in Karachi- Pakistan

November 16, 2021
Real-Time Spatial and Temporal Analysis of the Translocation of the Apoptosis-Inducing Factor in Cells
Ria Kits

Real-Time Spatial and Temporal Analysis of the Translocation of the Apoptosis-Inducing Factor in Cells

November 16, 2021
Development of Human Toxo IgG ELISA Kit, and False-Positivity of Latex Agglutination Test for the Diagnosis of Toxoplasmosis
Ria Kits

Development of Human Toxo IgG ELISA Kit, and False-Positivity of Latex Agglutination Test for the...

October 5, 2021
Ria Kits

Targeted mass spectrometry for monitoring of neural differentiation

October 5, 2021
Analyze the SUMOylation of IKK γ/NEMO During Genotoxic Stress
Ria Kits

Analyze the SUMOylation of IKK γ/NEMO During Genotoxic Stress

October 5, 2021
1234Page 1 of 4
  • Application of Metabolism Assay Kits in Drug Screening and Toxicology Studies
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  • The Importance of PCR Qualitative Positive Controls in Diagnostic Assays: Ensuring Reliable Results
  • Optimizing Giemsa Stain PR80 for Accurate Cytogenetic Analysis in Cancer Diagnostics
  • Standardization and Optimization of oxLDL-R1 ELISA for Cardiovascular Disease Biomarker Detection
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POPULAR POSTS

Multifocal tuberculosis simulating multimetastatic colon cancer in an immunocompetent black African patient: a case report

Inhalable nanoparticles delivery targeting alveolar macrophages for the treatment of pulmonary tuberculosis

September 25, 2021
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L-amino acid oxidase from snake venom: Biotransformation and induction of apoptosis in human colon cancer...

November 16, 2021
Diagnosis of intra-thoracic tuberculosis (ITTB) in children is difficult due to the paucibacillary nature of the disease, the challenge in collecting appropriate specimens, and the low sensitivity of smear microscopy and culture. Culture and Xpert MTB/RIF provide higher diagnostic yield in presumptive TB in adults than in children. Current study was designed to understand poor yield of diagnostic assays in children. Children with presumptive ITTB were subjected to gastric aspirates and induced sputum twice. Samples were tested by Ziehl-Neelsen stain, Xpert MTB/RIF-assay, and MGIT-960 culture. Subjects were grouped as Confirmed, Unconfirmed, and Unlikely TB, and classified as progressive primary disease (PPD, lung parenchymal lesion), and primary pulmonary complex (PPC, hilar lymphadenopathy) on chest X-ray. Of children with culture-positive TB 51/394 (12.9%), culture-negative TB 305 (77.4%), and unlikely TB 38 (9.6%), 9 (2.3%) were smear positive, while 95 (24.1%) were Xpert-MTB/RIF positive. Xpert-MTB/RIF detected 40/51 culture confirmed cases (sensitivity 78.4% and NPV 96.3%). Culture was positive in more children presenting as PPD (p < 0.04). In culture-negative TB group, Xpert positivity was seen in 31% of those with PPD and 11.9% in those with PPC (p < 0.001). Conclusion: Xpert-MTB/RIF improved diagnosis by 2-fold and increased detection of MDR-TB. Both liquid culture and Xpert-MTB/RIF gave higher yield in children with lung parenchymal lesions. Children with hilar lymphadenopathy without active lung parenchymal lesions had poor diagnostic yield even with sensitive nucleic acid amplification tests, due to paucibacillary/localized disease, suggesting possible utility of invasively collected samples in early diagnosis and treatment.

Diagnosis of intra-thoracic tuberculosis (ITTB) in children is difficult due to the paucibacillary nature of...

September 28, 2021

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