Alzheimer’s Illness (AD) and Kind 2 Diabetes (T2D) share a standard hallmark of insulin resistance. Reportedly, two non-canonical Receptor Tyrosine Kinases (RTKs), ALK and RYK, each targets of the identical micro RNA miR-1271, exhibit vital and constant purposeful downregulation in autopsy AD and T2D tissues. By the way, each have Grb2 as a standard downstream adapter and NOX4 as a standard ROS producing issue.
Right here we present that Grb2 and NOX4 play essential roles in lowering the severity of each the ailments. The examine demonstrates that the abundance of Grb2 in degenerative situations, together with NOX4, reverse cytoskeletal degradation by counterbalancing the community of small GTPases. PAX4, a transcription issue for each Grb2 and NOX4, emerges as the important thing hyperlink between the widespread pathways of AD and T2D.
Downregulation of each ALK and RYK by means of miR-1271, elevates the PAX4 degree by lowering its suppressor ARX through Wnt/b-Catenin signaling. For the primary time, this examine brings collectively RTKs past Insulin Receptor (IR) household, transcription issue PAX4 and each AD and T2D pathologies on a standard regulatory platform.
Function of receptor tyrosine kinases mediated sign transduction pathways in tumor development and angiogenesis-New perception and futuristic imaginative and prescient
Vital progress has been made prior to now twenty years in the direction of the understanding of the fundamental mechanisms underlying most cancers development and angiogenesis. On this context, receptor tyrosine kinases (RTKs) play a pivotal position in cell proliferation, differentiation, development, motility, invasion, and angiogenesis, all of which contribute to tumor development and development.
Mutations in RTKs result in irregular sign transductions in a number of pathways corresponding to Ras-Raf, MEK-MAPK, PI3K-AKT and mTOR pathways, which impacts protein translation and a variety of organic features together with cell proliferation, survival, migration and vascular permeability.
Rising proof demonstrates that a number of kinases are concerned in angiogenesis together with RTKs corresponding to vascular endothelial development issue, platelet derived development issue, epidermal development issue, insulin-like development factor-1, macrophage colony-stimulating issue, nerve development issue, fibroblast development issue, Hepatocyte Development issue, Tie 1 & 2, Tek, Flt-3, Flt-Four and Eph receptors.
Overactivation of RTKs and its downstream regulation is implicated in tumor initiation and angiogenesis, which represents one of many hallmarks of most cancers. This assessment discusses the position of RTKs, PI3K, and mTOR, their involvement and their implication in pro-oncogenic mobile processes and angiogenesis with efficient approaches and newly accepted medicine to inhibit their extreme motion.
Ebola virus triggers receptor tyrosine kinase-dependent signaling to advertise the supply of viral particles to entry-conducive intracellular compartments
Filoviruses, such because the Ebola virus (EBOV) and Marburg virus (MARV), are causative brokers of sporadic outbreaks of hemorrhagic fevers in people. To contaminate cells, filoviruses are internalized through macropinocytosis and visitors by means of the endosomal pathway the place host cathepsin-dependent cleavage of the viral glycoproteins happens.
Subsequently, the cleaved viral glycoprotein interacts with the late endosome/lysosome resident host protein, Niemann-Choose C1 (NPC1). This interplay is hypothesized to set off viral and host membrane fusion, which ends up in the supply of the viral genome into the cytoplasm and subsequent initiation of replication. Some research recommend that EBOV viral particles activate signaling cascades and host-trafficking elements to advertise their localization with host elements which might be important for entry.
Nonetheless, the mechanism by means of which these activating indicators are initiated stays unknown. By screening a kinase inhibitor library, we discovered that receptor tyrosine kinase inhibitors potently block EBOV and MARV GP-dependent viral entry. Inhibitors of epidermal development issue receptor (EGFR), tyrosine protein kinase Met (c-Met), and the insulin receptor (InsR)/insulin like development issue 1 receptor (IGF1R) blocked filoviral GP-mediated entry and prevented development of replicative EBOV in Vero cells.
Moreover, inhibitors of c-Met and InsR/IGF1R additionally blocked viral entry in macrophages, the first targets of EBOV an infection. Curiously, whereas the c-Met and InsR/IGF1R inhibitors interfered with EBOV trafficking to NPC1, virus supply to the receptor was not impaired within the presence of the EGFR inhibitor.
As a substitute, we noticed that the NPC1 optimistic compartments had been phenotypically altered and rendered incompetent to allow viral entry. Regardless of their completely different mechanisms of motion, all three RTK inhibitors examined inhibited virus-induced Akt activation, offering a attainable clarification for a way EBOV might activate signaling pathways throughout entry. In sum, these research strongly recommend that receptor tyrosine kinases provoke signaling cascades important for environment friendly post-internalization entry steps.
Analyzing Signaling Pathways Utilizing Antibody Arrays
Cell signaling is comprised of advanced networks that regulate homeostasis and human ailments. The analyses of such pathways would enhance our understanding of illness pathology and direct drug growth. Nonetheless, it stays an ideal problem to check pathways utilizing conventional strategies.
We developed a high-throughput sandwich-based antibody array expertise for the simultaneous detection of a number of targets, able to figuring out the relative expression ranges or phosphorylation ranges of main signaling pathway proteins. This array-based system encompasses a nitrocellulose membrane or glass slide strong assist, noticed with antibodies concentrating on key proteins of main signaling pathways, together with RTK, EGFR, MAPK, AKT, apoptosis, TGFb, JAK/STAT, NFkB, and insulin receptor pathways.
We employed these antibody arrays to analyze how the anti-cancer medicine, camptothecin and phorbol 12-myristate 13-acetate (PMA), alter protein phosphorylation in Jurkat and HeLa cells, respectively. Our array knowledge recommend that camptothecin therapy induced DNA double-strand breaks in Jurkat cells and activated the DNA injury pathways ATM and Chk2, which then additional induced apoptosis by means of caspase Three and PARP. PMA induced the MAPK pathway in HeLa cells by means of the activation of ERK, CREB, and RSK1.
These array outcomes are in line with earlier research utilizing conventional strategies and had been validated with Western blotting. Our research display that pathway antibody arrays present a speedy, environment friendly, and multiplexed strategy for profiling phosphorylated proteins.
In silico evaluation of antidiabetic potential of phenolic compounds from blue corn (Zea mays L.) and black bean (Phaseolus vulgaris L.).
The rising curiosity in bioactive compounds, particularly in polyphenols, is because of their abundance within the human weight loss program and doubtlessly optimistic results on well being. The consumption of polyphenols has been proven to own anti-diabetic properties by stopping insulin resistance or insulin secretion by means of completely different signaling pathways, this impact is related to their capability to exert genomic modulations.
A number of research have advised that polyphenols may additionally bind to mobile proteins and modulate their exercise, nevertheless, the mechanisms of motion underlying their useful results are advanced and will not be totally understood.
The purpose of this work was to characterize phenolic compounds current in blue corn and black bean extracts in addition to determine their potential interactions with goal proteins concerned in diabetes pathogenesis utilizing in silico strategy. Complete polyphenols content material of each blue corn and black beans was recognized utilizing UPLC-ESI/qTOF/MS and quantified by colorimetric assays.
On this work we recognized twenty-eight phenolic compounds within the extracts, primarily anthocyanins, flavonols, hydroxycinamic acids, dihydroxybenzoic acids, flavones, isoflavones, and flavanols. Interactome of those compounds with 13 goal proteins concerned in sort 2 diabetes mellitus was carried out in-silico. In complete, 312 bioactive compounds/protein interplay analyses had been acquired.
Molecular docking outcomes highlighted that 9 of the highest ten interactions correspond to anthocyanins, cyanidin 3-glucoside with 11β-HS, GFAT, PPARG; delphinidin 3-glucoside with 11β-HS, GFAT, PTP and RTKs; and petunidin 3-glucoside with 11β-HS and PTP. These proteins are concerned in mechanisms regulating features corresponding to irritation, insulin resistance, oxidative stress, glucose and lipid metabolism.
JAK/STAT Pathway Sampler Kit |
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| E051015 | EnoGene | 5x50ug | EUR 475 |
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Description: Available in various conjugation types. |
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Hh Signaling Pathway Antagonist |
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| 1659-1 | Biovision | each | EUR 216 |
Human Calcium Regulation Pathway |
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| HCAR-I | Real Time Primers | 1 set | EUR 657.6 |
β -Catenin Pathway Sampler Kit |
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| E051023 | EnoGene | 5x50ug | EUR 475 |
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Description: Available in various conjugation types. |
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Notch1 Pathway Reporter Kit (Human) |
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| 79503 | BPS Bioscience | 500 rxns. | EUR 650 |
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Description: Notch Pathway Reporter kit is designed for monitoring the activity of the Notch signaling pathway in cultured cells. The kit contains a transfection-ready expression vector for Human NOTCH1 that has a deletion of the entire extracellular domain, leaving the transmembrane and intracellular domains intact (Notch1DE). Inside the cells, the NOTCH1 DE is cleaved by γ-secretase and active NOTCH1 NICD is released into the nucleus. The kit also contains CSL (CBF1/RBP-Jk) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized CSL responsive elements upstream of a minimal promoter. The CSL (CBF1/RBP-Jk) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity._x000D_This kit contains the expression vector for Human Notch1DE. We also offer the Mouse Notch1DE expression vector (BPS Bioscience #60509), as well as a Mouse Notch1 NICD expression vector, which is constitutively active without processing by γ-secretase (BPS Bioscience #79504)._x000D_ |
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Phospho-AKT Pathway Sampler Kit |
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| E051002 | EnoGene | 5x50ug | EUR 700 |
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Description: Available in various conjugation types. |
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Phospho-PLC Pathway Sampler Kit |
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| E051020 | EnoGene | 5x50ug | EUR 700 |
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Description: Available in various conjugation types. |
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Phospho-MAPK Pathway Sampler Kit |
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| E051018 | EnoGene | 5x50ug | EUR 700 |
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Description: Available in various conjugation types. |
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Phospho-NFκB Pathway Sampler Kit |
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| E051022 | EnoGene | 5x50ug | EUR 700 |
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Description: Available in various conjugation types. |
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TOR Signaling Pathway Detection Set |
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| PSI-1805 | ProSci | 1 Set | EUR 1287.6 |
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Description: The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle progression through its ability to integrate signals from nutrient levels and growth factors. TOR regulation is accomplished through a network of various activators and repressors. It is phosphorylated by Akt, whose activity is indirectly inhibited by the lipid phosphatase PTEN. TOR is normally associated with the regulatory proteins RAPTOR, a scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation, and GΒL, which stimulates TOR’s kinase activity towards downstream proteins. It is further regulated by the proteins Rheb, TSC1 and TSC2, which act to modulate TOR activity. The downstream targets of TOR are thought to be the ribosomal protein S6 kinases and the eukaryotic initiation factor 4E binding proteins (4EBPs) whose activation leads to increased protein translation and cell growth.;;For images please see PDF data sheet |
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Human Cancer Pathway Primer Library |
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| HCAN-I | Real Time Primers | 1 set | EUR 657.6 |
Human Hippo Signaling Primer Pathway |
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| HHPO-I | Real Time Primers | 1 set | EUR 657.6 |
FOXO Reporter Kit (PI3K/AKT Pathway) |
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| 60643 | BPS Bioscience | 500 rxns. | EUR 565 |
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Description: The FOXO Reporter kit is designed to monitor activity of the PI3K/AKT signaling pathway and the transcriptional activity of FOXO proteins in cultured cells. The kit contains the transfection-ready FOXO3 expression vector and the FOXO luciferase reporter vector, which is a PI3K/Akt pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimers of the FOXO responsive element located upstream of a minimal promoter. The FOXO reporter is premixed with a constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal control for the transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector also contains the firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity. |
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Phospho-Integrin Pathway Sampler Kit |
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| E051026 | EnoGene | 5x50ug | EUR 700 |
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Description: Available in various conjugation types. |
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Notch1 NICD Pathway Reporter Kit (Mouse) |
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| 79504 | BPS Bioscience | 500 rxns. | EUR 590 |
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Description: Notch Pathway Reporter kit is designed for monitoring the activity of the Notch signaling pathway in cultured cells. The kit contains a transfection-ready expression vector for Mouse NOTCH1 that has a deletion of the entire extracellular domain and transmembrane region (NOTCH1 NICD). Inside the cells, the NOTCH1 NICD is constitutively localized into the nucleus without needing to be cleaved by γ-secretase. The kit also contains CSL (CBF1/RBP-Jκ) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized CSL responsive elements upstream of a minimal promoter. The CSL (CBF1/RBP-Jκ) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity._x000D_This kit contains the expression vector for Mouse Notch1 NICD, which does not require γ-secretase processing to become active. We also offer the Mouse Notch1DE expression vector (BPS Bioscience #60509), as well as a Human Notch1DE expression vector (BPS Bioscience #79503), which are dependent on γ-secretase activity to become active. |
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Myc Reporter Kit (Myc Signaling Pathway) |
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| 60519 | BPS Bioscience | 500 rxns. | EUR 625 |
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Description: The Myc Pathway Reporter kit is designed for monitoring the activity of the Myc signaling pathway in cultured cells. The kit contains a transfection-ready expression vector for c- Myc and Myc luciferase reporter vector. Inside the cells, c-Myc will bind to Max, translocate to the nucleus, and induce expression of the Myc luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized Myc responsive elements located upstream of a minimal promoter. The Myc reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The kit also includes a non-inducible firefly luciferase vector premixed with constitutivelyexpressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway-specific effects and background luciferase activity. |
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Phospho-JAK/STAT Pathway Sampler Kit |
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| E051016 | EnoGene | 5x50ug | EUR 700 |
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Description: Available in various conjugation types. |
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ARE Reporter Kit (Nrf2 Antioxidant Pathway ) |
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| 60514 | BPS Bioscience | 500 rxns. | EUR 650 |
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Description: The ARE Reporter kit is designed for monitoring the activity of the Nrf2 antioxidant pathway in cultured cells. The kit contains a transfection-ready ARE luciferase reporter vector, which is an Nrf2 pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized ARE responsive elements located upstream of a minimal promoter. The ARE reporter is premixed with a constitutively expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. This negative control is critical to determining pathway-specific effects and background luciferase activity. |
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Phospho-β-Catenin Pathway Sampler Kit |
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| E051024 | EnoGene | 5x50ug | EUR 700 |
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Description: Available in various conjugation types. |
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G-protein pathway suppressor 2 Antibody |
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| 20-abx137433 | Abbexa |
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Tissue Factor Pathway Inhibitor 2 Protein |
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| 20-abx263563 | Abbexa |
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STAT3 Reporter Kit (STAT3 Signaling Pathway) |
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| 79730 | BPS Bioscience | 500 rxns. | EUR 640 |
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Description: The STAT3 Reporter kit is designed for monitoring the activity of the STAT3 signaling pathway in cultured cells. The kit contains transfection-ready STAT3 luciferase reporter vector. This reporter contains a firefly luciferase gene under the control of STAT3-responsive element located upstream of a minimal promoter. The STAT3 reporter is premixed with constitutively expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency._x000D_ The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity. |
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Human Tissue factor pathway inhibitor (TFPI) |
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| 1-CSB-EP023437HU | Cusabio |
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Description: Recombinant Human Tissue factor pathway inhibitor(TFPI),partial expressed in E.coli |
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Tissue Factor Pathway Inhibitor Rabbit mAb |
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| E2R27344 | EnoGene | 100ul | EUR 255 |
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Description: Available in various conjugation types. |
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Tissue Factor Pathway Inhibitor Rabbit mAb |
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| E2R27346 | EnoGene | 100ul | EUR 255 |
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Description: Available in various conjugation types. |
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SRE Reporter - HEK293 Cell Line (ERK Pathway) |
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| 60406 | BPS Bioscience | 2 vials | EUR 2505 |
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Description: The SRE Reporter - HEK293 cell line is designed for monitoring the activity of the JAK/STAT signaling pathway. The SRE Reporter - HEK293 cell line contains a firefly luciferase gene under the control of SRE responsive elements stably integrated into HEK293 cells, resulting in an ERK pathway-responsive reporter cell line. |
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Rabbit Tissue factor pathway inhibitor (TFPI) |
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| 1-CSB-BP023437RB | Cusabio |
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Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in Baculovirus |
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Rabbit Tissue factor pathway inhibitor (TFPI) |
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| 1-CSB-YP023437RB | Cusabio |
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Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in Yeast |
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Rabbit Tissue factor pathway inhibitor (TFPI) |
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| 1-CSB-EP023437RB | Cusabio |
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Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in E.coli |
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Rabbit Tissue factor pathway inhibitor (TFPI) |
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| 1-CSB-MP023437RB | Cusabio |
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Description: Recombinant Rabbit Tissue factor pathway inhibitor(TFPI) expressed in Mammalian cell |
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TCF/LEF Reporter Kit (Wnt Signaling Pathway) |
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| 60500 | BPS Bioscience | 500 rxns. | EUR 655 |
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Description: The TCF/LEF Reporter kit is designed for monitoring the activity of Wnt / β-catenin signaling pathway in the cultured cells. The kit contains transfection-ready TCF/LEF luciferase reporter vector, which is a Wnt pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized TCF/LEF responsive element located upstream of a minimal promoter. The TCF/LEF reporter is premixed with constitutively-expressing Renilla luciferase vector that serves as internal control for transfection efficiency. _x000D_The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity. _x000D_ |
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Tissue Factor Pathway Inhibitor (TFPI) Protein |
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| 20-abx261170 | Abbexa |
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SRE Reporter Kit (MAPK/ERK Signaling Pathway ) |
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| 60511 | BPS Bioscience | 500 rxns. | EUR 595 |
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Description: The SRE Reporter Kit is designed for monitoring the activity of the ERK signaling pathway and the transcriptional activity of SRF in cultured cells. The kit contains a transfection-ready SRE luciferase reporter vector, which is an ERK pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized SRE responsive elements located upstream of a minimal promoter. The SRE reporter is premixed with a constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity. |
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Tissue Factor Pathway Inhibitor (TFPI) Antibody |
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| abx238634-100ug | Abbexa | 100 ug | EUR 661.2 |
Tissue Factor Pathway Inhibitor (TFPI) Antibody |
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| 20-abx103325 | Abbexa |
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Tissue Factor Pathway Inhibitor (TFPI) Antibody |
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| 20-abx103326 | Abbexa |
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Tissue Factor Pathway Inhibitor (TFPI) Antibody |
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| 20-abx103328 | Abbexa |
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Tissue Factor Pathway Inhibitor (TFPI) Antibody |
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| 20-abx103329 | Abbexa |
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Tissue Factor Pathway Inhibitor (TFPI) Antibody |
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| 20-abx104947 | Abbexa |
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Tissue Factor Pathway Inhibitor (TFPI) Antibody |
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| 20-abx174804 | Abbexa |
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Tissue Factor Pathway Inhibitor (TFPI) Antibody |
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| 20-abx116110 | Abbexa |
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Tissue Factor Pathway Inhibitor (TFPI) Antibody |
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| 20-abx129517 | Abbexa |
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Tissue Factor Pathway Inhibitor (TFPI) Antibody |
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| 20-abx132084 | Abbexa |
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Tissue Factor Pathway Inhibitor (TFPI) Antibody |
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| 20-abx132085 | Abbexa |
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Tissue Factor Pathway Inhibitor (TFPI) Antibody |
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| 20-abx132086 | Abbexa |
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Tissue Factor Pathway Inhibitor (TFPI) Antibody |
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| 20-abx323593 | Abbexa |
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Tissue Factor Pathway Inhibitor (TFPI) Antibody |
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| abx433382-200ul | Abbexa | 200 ul | EUR 460.8 |
Tissue Factor Pathway Inhibitor (TFPI) Antibody |
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| 20-abx001368 | Abbexa |
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ISRE Reporter Kit (JAK/STAT Signaling Pathway) |
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| 60613 | BPS Bioscience | 500 rxns. | EUR 565 |
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Description: The ISRE Reporter kit is designed for monitoring the activity of Type I interferon-induced JAK/STAT signaling pathway in the cultured cells. The kit contains transfection-ready ISRE luciferase reporter vector, which is a JAK/STAT pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized ISRE responsive element located upstream of a minimal promoter. The ISRE reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The noninducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity. |
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SBE Reporter Kit (TGFβ/SMAD signaling pathway) |
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| 60654 | BPS Bioscience | 500 rxns. | EUR 595 |
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Description: The SBE Reporter kit is designed for monitoring the activity of TGFβ/SMAD signaling pathway in the cultured cells. The kit contains transfection-ready SBE luciferase reporter vector, which is a TGFβ pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized SBE responsive element located upstream of a minimal promoter. The SBE reporter is premixed with constitutively-expressing Renilla luciferase vector that serves as internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as negative control. The noninducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity. |
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Dog Tissue factor pathway inhibitor ELISA kit |
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| E08T0509-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Canine Tissue factor pathway inhibitor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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In conclusion, this work gives a prediction of the potential molecular mechanism of black bean and blue corn polyphenols, particularly anthocyanins and will represent new pathways by which compounds exert their antidiabetic advantages.
