The receptor-interacting protein kinase 4 (RIPK4) performs an essential function within the growth and upkeep of varied tissues together with pores and skin, however its function in melanoma has not been reported. Utilizing patient-derived cell strains and medical samples, we present that RIPK4 is expressed in melanomas at totally different ranges.
This heterogenous expression, along with very low stage of RIPK4 in melanocytes, signifies that the function of this kinase in melanoma is context-dependent. Whereas the evaluation of microarray information has revealed no simple correlation between the stage of melanoma development and RIPK4 expression in vivo, comparatively excessive ranges of RIPK4 are in metastatic melanoma cell strains.
RIPK4 down-regulation by siRNA resulted within the attenuation of invasive potential as assessed by time-lapse video microscopy, wound-healing and transmigration assays. These results had been accompanied by decreased stage of pro-invasive proteins reminiscent of MMP9, MMP2, and N-cadherin. Incubation of melanoma cells with phorbol ester (PMA) elevated PKC-1β stage and hyperphosphorylation of RIPK4 leading to degradation of RIPK4.
Curiously, incubation of cells with PMA for brief and lengthy durations revealed that cell migration is managed by the NF-κB signaling in a RIPK4-dependent (RIPK4excessive) or impartial (RIPK4low) method relying on cell origin (distant or lymph node metastasis) or phenotype (mesenchymal or epithelial).h

The Laminin Receptors Basal Cell Adhesion Molecule/Lutheran and Integrin α7β1 on Human Hematopoietic Stem Cells

Within the grownup organism, hematopoietic stem and progenitor cells (HSPC) reside within the bone marrow (BM) in specialised hematopoietic stem cell niches of which the extracellular matrix (ECM) is an integral part. Laminins (LM) are a household of heterotrimeric ECM molecules of which primarily relations containing an α4 or α5 chain are expressed in cells from BM niches and concerned in HSPC homing and proliferation. Numerous integrin and non-integrin laminin receptors have been recognized and characterised.
Amongst these, the integrins α6β1 and α3β1 had been reported to be strongly expressed on human and mouse HSPC. Within the current examine, we concentrate on two additional particular laminin receptors, specifically integrin α7β1 and basal cell adhesion molecule/Lutheran (BCAM/Lu). Utilizing RT-PCR analyses, immunofluorescence staining, immunoblotting and move cytometry, we present that each are strongly expressed by human lineage-negative CD34 + HSPC.

Human Cluster Of Differentiation 34 (CD34) ELISA Kit

RD-CD34-Hu-48Tests Reddot Biotech 48 Tests 500 EUR

Human Cluster Of Differentiation 34 (CD34) ELISA Kit

RD-CD34-Hu-96Tests Reddot Biotech 96 Tests 692 EUR

Mouse Cluster Of Differentiation 34 (CD34) ELISA Kit

RD-CD34-Mu-48Tests Reddot Biotech 48 Tests 511 EUR

Mouse Cluster Of Differentiation 34 (CD34) ELISA Kit

RD-CD34-Mu-96Tests Reddot Biotech 96 Tests 709 EUR

Human Cluster Of Differentiation 34 (CD34) ELISA Kit

DLR-CD34-Hu-48T DL Develop 48T 498 EUR

Human Cluster Of Differentiation 34 (CD34) ELISA Kit

DLR-CD34-Hu-96T DL Develop 96T 647 EUR

Mouse Cluster Of Differentiation 34 (CD34) ELISA Kit

DLR-CD34-Mu-48T DL Develop 48T 508 EUR

Mouse Cluster Of Differentiation 34 (CD34) ELISA Kit

DLR-CD34-Mu-96T DL Develop 96T 661 EUR

Human Cluster Of Differentiation 34 (CD34) ELISA Kit

RDR-CD34-Hu-48Tests Reddot Biotech 48 Tests 522 EUR

Human Cluster Of Differentiation 34 (CD34) ELISA Kit

RDR-CD34-Hu-96Tests Reddot Biotech 96 Tests 724 EUR

Mouse Cluster Of Differentiation 34 (CD34) ELISA Kit

RDR-CD34-Mu-48Tests Reddot Biotech 48 Tests 534 EUR

Mouse Cluster Of Differentiation 34 (CD34) ELISA Kit

RDR-CD34-Mu-96Tests Reddot Biotech 96 Tests 742 EUR

CD34

PR27319 Neuromics 5 ug 191 EUR

CD34

34A-100T ImmunoStep 100 test 479 EUR

CD34

34AC750-100T ImmunoStep 100 test 765 EUR

CD34

34F-100T ImmunoStep 100 test 349 EUR

CD34

34PE-100T ImmunoStep 100 test 427 EUR

CD34

34PP-100T ImmunoStep 100 test 492 EUR

CD34

34PP5.5-100T ImmunoStep 100 test 531 EUR

CD34

MO34A(V100) ImmunoStep 100 ug 50 EUR

CD34

MO34A(V25) ImmunoStep 25 ug 50 EUR

CD34

MO34B(V100) ImmunoStep 100 ug 50 EUR

CD34

MO34B(V25) ImmunoStep 25 ug 50 EUR

CD34

MO34PE(V200) ImmunoStep 100 ug 50 EUR

CD34

MO34PE(V50) ImmunoStep 25 ug 50 EUR

CD34

MO34PU(V50) ImmunoStep 100 ug 50 EUR

CD34

MO34PU(V500) ImmunoStep 500 ug 50 EUR

CD34 antigen (CD34) polyclonal antibody

ABP-PAB-00010 Allele Biotech 100 ug Ask for price

CD34 Antibody

CSB-PA269036- Cusabio 335 EUR

CD34 Antibody

CSB-PA269036-100ul Cusabio 100ul 316 EUR

CD34 Antibody

1-CSB-PA001451 Cusabio
  • 222.00 EUR
  • 195.00 EUR
  • 100ug
  • 50ug

CD34 Antibody

CSB-PA004926KA01HU- Cusabio 335 EUR

CD34 Antibody

CSB-PA004926KA01HU-100ul Cusabio 100ul 389 EUR

CD34 Antibody

1-CSB-PA10509A0Rb Cusabio
  • 317.00 EUR
  • 335.00 EUR
  • 100ug
  • 50ug

CD34/HPCA1

MO18004 Neuromics 100 ul 483 EUR

CD34/HPCA1

MO20026 Neuromics 100 ul 252 EUR

CD15/CD34

15F34PE-50T ImmunoStep 50 test 436.1 EUR

CD34 Antibody

21469-100ul SAB 100ul 252 EUR

CD34 Antibody

21469-50ul SAB 50ul 187 EUR

CD34 Antibody

35493-100ul SAB 100ul 390 EUR

CD34 Antibody

35494-100ul SAB 100ul 390 EUR

CD34 siRNA

20-abx911013 Abbexa
  • 551.00 EUR
  • 732.00 EUR
  • 15 nmol
  • 30 nmol

CD34 siRNA

20-abx911014 Abbexa
  • 551.00 EUR
  • 732.00 EUR
  • 15 nmol
  • 30 nmol

CD34 Protein

20-abx262149 Abbexa
  • 5311.00 EUR
  • 328.00 EUR
  • 230.00 EUR
  • 1 mg
  • 20 ug
  • 5 ug

CD34 Protein

20-abx263541 Abbexa
  • 829.00 EUR
  • 411.00 EUR
  • 537.00 EUR
  • 10 ug
  • 2 µg
  • 5 ug

CD34 Antibody

20-abx242939 Abbexa
  • 467.00 EUR
  • 328.00 EUR
  • 100 ul
  • 50 ul

CD34 antibody

38153-100ul SAB 100ul 252 EUR

CD34 Antibody

43792-100ul SAB 100ul 252 EUR

CD34 Antibody

48740-100ul SAB 100ul 333 EUR

CD34 Antibody

48740-50ul SAB 50ul 239 EUR

CD34 Antibody

ABD6139 Lifescience Market 100 ug 438 EUR

CD34 Antibody

ABF4036 Lifescience Market 100 ug 438 EUR

CD34 Antibody

ABF5149 Lifescience Market 100 ug 438 EUR

CD34 Antibody

DF6139 Affbiotech 200ul 304 EUR

Anti-CD34

DB151RTU-15 DB Biotech 15 ml 347 EUR

Anti-CD34

DB151RTU-7 DB Biotech 7 ml 228 EUR

Anti-CD34

DB-151-0.1 DB Biotech 100 μl 209 EUR

Anti-CD34

DB-151-0.2 DB Biotech 200 μl 293 EUR

Anti-CD34

DB-151-0.5 DB Biotech 500 μl 379 EUR

Anti-CD34

DB-151-1 DB Biotech 1 ml 602 EUR

Anti-CD34

DB-151-RTU-15 DB Biotech 15 ml 347 EUR

Anti-CD34

DB-151-RTU-7 DB Biotech 7 ml 228 EUR

CD34 Antibody

BF0423 Affbiotech 200ul 376 EUR

CD34 Peptide

43-091P ProSci 0.1 mg 338 EUR

CD34 antibody

10R-6385 Fitzgerald 100 ug 203 EUR

CD34 antibody

10R-7653 Fitzgerald 50 ug 457 EUR

CD34 antibody

10R-1923 Fitzgerald 100 ul 435 EUR

CD34 antibody

10R-2342 Fitzgerald 1 mL 589 EUR

CD34 antibody

70R-10219 Fitzgerald 50 ug 467 EUR

CD34 antibody

70R-10220 Fitzgerald 50 ug 467 EUR

CD34 Antibody

AF4036 Affbiotech 200ul 376 EUR

CD34 Antibody

AF5149 Affbiotech 200ul 304 EUR

CD34 antibody

20R-2517 Fitzgerald 50 ug 281 EUR

CD34 antibody

70R-13876 Fitzgerald 100 ug 305 EUR

anti-CD34

YF-PA27190 Abfrontier 50 ug 363 EUR

anti-CD34

YF-PA23393 Abfrontier 50 ul 334 EUR

CD34 Antibody

DF16119 Affbiotech 100ul 350 EUR
Remedy with function-blocking antibodies towards BCAM/Lu neither inhibits the robust adhesive interplay of CD34 + HSPC with LM-511/LM-521 nor the LM-511/LM-521 mediated modifications in CD34 + HSPC proliferation, however nonetheless, influences the cytokine-induced differentiation of HSPC in colony formation assays.
As well as, stromal-derived issue (SDF) 1α-mediated transmigration of CD34 + HSPC by means of an endothelial cell layer was successfully diminished by BCAM/Lu antibodies, suggesting a direct involvement of BCAM/Lu within the migration course of. This examine signifies that each laminin receptors newly recognized on human CD34 + HSPC ought to be considered in future research.

CX3CL1(+) Microparticles-Induced MFG-E8 Enhances Apoptotic Cell Clearance by Alveolar Macrophages

Through the decision part of acute lung damage, apoptotic cells launch CX3CL1 as a “find-me” sign to draw alveolar macrophage transmigration towards apoptotic cells for phagocytosis. Nevertheless, it’s nonetheless not clear whether or not CX3CL1 has pro-phagocytic exercise on alveolar macrophage.
On this examine, we investigated the function of apoptotic NB4 cells-derived CX3CL1(+) microparticles (apo-MP) on the phagocytic exercise of NR8383 cells. We exhibit that exogenous CX3CL1 and apo-MP enhanced the phagocytic exercise of NR8383 cells in a CX3 CR1-dependent method. The apo-MP-enhanced phagocytic exercise on NR8383 was attenuated when apo-MP and NR8383 cells had been pre-treated with anti-CX3CL1 antibodies and anti-CX3CR1 antibody, respectively, earlier than incubating each for phagocytic assay.
Additional research exhibit that exogenous CX3CL1 and apo-MP additionally enhanced NR8383 cells of their floor expression and launch of MFG-E8 in a CX3CR1 dependent method. The improved phagocytic exercise of CX3CL1-treated NR8383 cells was attenuated when NR8383 cells had been pre-treated with an anti-MFG-E8 antibody earlier than CX3CL1 therapy.

Human Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit

RD-CX3CL1-Hu-48Tests Reddot Biotech 48 Tests 478 EUR

Human Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit

RD-CX3CL1-Hu-96Tests Reddot Biotech 96 Tests 662 EUR

Mouse Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit

RD-CX3CL1-Mu-48Tests Reddot Biotech 48 Tests 372 EUR

Mouse Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit

RD-CX3CL1-Mu-96Tests Reddot Biotech 96 Tests 510 EUR

Rat Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit

RD-CX3CL1-Ra-48Tests Reddot Biotech 48 Tests 511 EUR

Rat Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit

RD-CX3CL1-Ra-96Tests Reddot Biotech 96 Tests 709 EUR

Human Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit

DLR-CX3CL1-Hu-48T DL Develop 48T 479 EUR

Human Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit

DLR-CX3CL1-Hu-96T DL Develop 96T 621 EUR

Mouse Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit

DLR-CX3CL1-Mu-48T DL Develop 48T 385 EUR

Mouse Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit

DLR-CX3CL1-Mu-96T DL Develop 96T 492 EUR

Rat Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit

DLR-CX3CL1-Ra-48T DL Develop 48T 508 EUR

Rat Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit

DLR-CX3CL1-Ra-96T DL Develop 96T 661 EUR

Human Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit

RDR-CX3CL1-Hu-48Tests Reddot Biotech 48 Tests 500 EUR

Human Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit

RDR-CX3CL1-Hu-96Tests Reddot Biotech 96 Tests 692 EUR

Mouse Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit

RDR-CX3CL1-Mu-48Tests Reddot Biotech 48 Tests 388 EUR

Mouse Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit

RDR-CX3CL1-Mu-96Tests Reddot Biotech 96 Tests 533 EUR

Rat Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit

RDR-CX3CL1-Ra-48Tests Reddot Biotech 48 Tests 534 EUR

Rat Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit

RDR-CX3CL1-Ra-96Tests Reddot Biotech 96 Tests 742 EUR

CX3CL1 Antibody

1-CSB-PA005564 Cusabio
  • 222.00 EUR
  • 195.00 EUR
  • 100ug
  • 50ug

CX3CL1 Antibody

1-CSB-PA006235GA01HU Cusabio
  • 597.00 EUR
  • 333.00 EUR
  • 150ul
  • 50ul

CX3CL1 Antibody

1-CSB-PA06087A0Rb Cusabio
  • 317.00 EUR
  • 335.00 EUR
  • 100ug
  • 50ug

CX3CL1 Antibody

1-CSB-PA092112 Cusabio
  • 317.00 EUR
  • 244.00 EUR
  • 100ul
  • 50ul

Fractalkine(CX3CL1)

GT15024 Neuromics 100 ug 526 EUR

CX3CL1 Antibody

24058-100ul SAB 100ul 390 EUR

CX3CL1 Antibody

36816-100ul SAB 100ul 252 EUR

CX3CL1 siRNA

20-abx913227 Abbexa
  • 551.00 EUR
  • 732.00 EUR
  • 15 nmol
  • 30 nmol

CX3CL1 siRNA

20-abx913228 Abbexa
  • 551.00 EUR
  • 732.00 EUR
  • 15 nmol
  • 30 nmol

CX3CL1 siRNA

20-abx901345 Abbexa
  • 551.00 EUR
  • 732.00 EUR
  • 15 nmol
  • 30 nmol

CX3CL1 Antibody

DF12376 Affbiotech 200ul 304 EUR

CX3CL1 Antibody

2099-002mg ProSci 0.02 mg 171.82 EUR

CX3CL1 Antibody

2099-01mg ProSci 0.1 mg 436.42 EUR

CX3CL1 Peptide

2099P ProSci 0.05 mg 164.75 EUR

CX3CL1 antibody

70R-16678 Fitzgerald 50 ul 435 EUR

FKN/CX3CL1/ Rat FKN/ CX3CL1 ELISA Kit

ELA-E0040r Lifescience Market 96 Tests 886 EUR

CX3CL1 cloning plasmid

CSB-CL006235HU-10ug Cusabio 10ug 444 EUR

Human Fractalkine (CX3CL1)

1-CSB-EP006235HU(F) Cusabio
  • 380.00 EUR
  • 214.00 EUR
  • 1309.00 EUR
  • 560.00 EUR
  • 873.00 EUR
  • 262.00 EUR
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug

Anti-CX3CL1 Antibody

PA1400 BosterBio 100ug/vial 294 EUR

Anti-CX3CL1 Antibody

PA1401 BosterBio 100ug/vial 334 EUR

Fractalkine, CX3CL1, human

RC314-12 Bio Basic 5ug 104.38 EUR

Fractalkine, CX3CL1, rat

RC354-12 Bio Basic 5ug 104.38 EUR

Fractalkine/CX3CL1, Human

HY-P7180 MedChemExpress 50ug 533 EUR

Anti-CX3CL1 Antibody

STJ500636 St John's Laboratory 100 µg 476 EUR

Anti-CX3CL1 antibody

STJ116131 St John's Laboratory 100 µl 277 EUR

Polyclonal CX3CL1 Antibody

APR06243G Leading Biology 0.1 mg 659 EUR

CX3CL1 Rabbit pAb

A14198-100ul Abclonal 100 ul 308 EUR

CX3CL1 Rabbit pAb

A14198-200ul Abclonal 200 ul 459 EUR

CX3CL1 Rabbit pAb

A14198-20ul Abclonal 20 ul 183 EUR

CX3CL1 Rabbit pAb

A14198-50ul Abclonal 50 ul 223 EUR

CX3CL1 Polyclonal Antibody

A55627 EpiGentek 100 µg 570.55 EUR

anti- CX3CL1 antibody

FNab02088 FN Test 100µg 505.25 EUR

anti- CX3CL1 antibody

FNab02089 FN Test 100µg 505.25 EUR

CX3CL1 Conjugated Antibody

C36816 SAB 100ul 397 EUR

CX3CL1 Blocking Peptide

DF12376-BP Affbiotech 1mg 195 EUR

CX3CL1 / fractalkine Peptide

43-578P ProSci 0.1 mg 338 EUR

CX3CL1 Recombinant Protein

96-859 ProSci 0.05 mg 542.75 EUR

Anti-CX3CL1 antibody

PAab02088 Lifescience Market 100 ug 355 EUR

Anti-CX3CL1 (1D6)

YF-MA15378 Abfrontier 100 ug 363 EUR

CX3CL1 Recombinant Protein

90-452 ProSci 25 ug 338 EUR

CX3CL1 Recombinant Protein

90-453 ProSci 100 ug 569 EUR

CX3CL1 Recombinant Protein

91-417 ProSci 0.05 mg 595.25 EUR

CX3CL1 Recombinant Protein

92-343 ProSci 0.05 mg 490.25 EUR

CX3CL1 Antibody, HRP conjugated

1-CSB-PA06087B0Rb Cusabio
  • 317.00 EUR
  • 335.00 EUR
  • 100ug
  • 50ug

CX3CL1 Antibody, FITC conjugated

1-CSB-PA06087C0Rb Cusabio
  • 317.00 EUR
  • 335.00 EUR
  • 100ug
  • 50ug

CX3CL1 Antibody, Biotin conjugated

1-CSB-PA06087D0Rb Cusabio
  • 317.00 EUR
  • 335.00 EUR
  • 100ug
  • 50ug

Sheep Cx3CL1 ELISA Kit

ESC0239 Abclonal 96Tests 521 EUR

Flt3-Ligand, CX3CL1, human

RC214-16 Bio Basic 2ug 104.38 EUR

Mouse Cx3CL1 ELISA Kit

EMC0239 Abclonal 96Tests 521 EUR

Anserini Cx3CL1 ELISA Kit

EAC0239 Abclonal 96Tests 521 EUR

Rabbit Cx3CL1 ELISA Kit

ERTC0239 Abclonal 96Tests 521 EUR

Monkey Cx3CL1 ELISA Kit

EMKC0239 Abclonal 96Tests 521 EUR

Porcine Cx3CL1 ELISA Kit

EPC0239 Abclonal 96Tests 521 EUR

Rat Cx3CL1 ELISA Kit

ERC0239 Abclonal 96Tests 521 EUR

Human Cx3CL1 ELISA Kit

EHC0239 Abclonal 96Tests 521 EUR

Canine Cx3CL1 ELISA Kit

ECC0239 Abclonal 96Tests 521 EUR

Chicken Cx3CL1 ELISA Kit

ECKC0239 Abclonal 96Tests 521 EUR

Goat Cx3CL1 ELISA Kit

EGTC0239 Abclonal 96Tests 521 EUR

Bovine Cx3CL1 ELISA Kit

EBC0239 Abclonal 96Tests 521 EUR
We conclude that apoptotic cell-derived CX3CL1(+) microparticles improve the phagocytic exercise of NR8383 cells by up-regulating their MFG-E8 as a bridge molecule, and these contribute to the formation of phagocytic synapses between apoptotic cells and alveolar macrophages for the next phagocytic clearance of apoptotic cells.
transmigration assay, Deciphering the Functional Role of RIPK4 in Melanoma

CD20 constructive CD8 T cells are a singular and transcriptionally-distinct subset of T cells with distinct transmigration properties

The presence of T cells which might be dimly constructive for the B cell marker CD20 is well-established in autoimmunity and correlates with illness severity in numerous illnesses. Additional, we beforehand recognized that the extent of CD20-positive T cells was three-fourfold elevated in ascites fluid of ovarian carcinoma sufferers, collectively suggesting a task in each autoimmunity and most cancers.
On this respect, therapy of autoimmune sufferers with the CD20-targeting antibody Rituximab has additionally been proven to focus on and deplete CD20-positive T cells, beforehand recognized as IFN-gamma producing, low proliferative, CD8 cytotoxic T cells with an effector reminiscence (EM) differentiation state. Nevertheless, the precise phenotype and relevance of CD20-positive T cells stays unclear.
Right here, we got down to establish the transcriptomic profile of CD20-positive T cells utilizing RNA sequencing. Additional, to achieve perception into potential useful properties of CD20 expression in T cells, CD20 was ectopically expressed on wholesome human T cells and phenotypic, useful, migratory and adhesive properties had been decided in vitro and in vivo. Collectively, these assays revealed a decreased transmigration and an enhanced adhesive profile mixed with an enhanced activation standing for CD20-positive T cells.

Transendothelial migration induces differential migration dynamics of leukocytes in tissue matrix

Leukocyte extravasation into infected tissue is a fancy course of that’s troublesome to seize as a complete in vitro. We employed a blood-vessel-on-a-chip mannequin by which endothelial cells had been cultured in a tube-like lumen in a collagen-1 matrix. The vessels are leak-tight, making a barrier for molecules and leukocytes. Addition of inflammatory cytokine TNF-α brought on vasoconstriction, actin remodelling and upregulation of ICAM-1.
Introducing leukocytes into the vessels allowed real-time visualisation of all totally different steps of the leukocyte transmigration cascade together with migration into the extracellular matrix. Particular person cell monitoring over time distinguished hanging variations in migratory behaviour between T-cells and neutrophils.

Assay Buffer, 60ML

X047-60ML 60ML
EUR 122

Assay Buffer, 28ML

X053-28ML 28ML
EUR 123

Assay Buffer, 55ML

X053-55ML 55ML
EUR 153

Assay Buffer, 60ML

X089-60ML 60ML
EUR 109

Assay Buffer, 50ML

X100-50ML 50ML
EUR 109

Assay Buffer, 50ML

X117-50ML 50ML
EUR 143

Lactose Assay Kit

MET-5001 100 assays
EUR 432
Description: The Lactose Assay Kit measures total lactose in milk based food products or biological samples such as blood or urine. Lactose is cleaved into glucose and galactose. Glucose is then oxidized, yielding hydrogen peroxide and D-gluconic acid. The hydrogen peroxide is detected by a fluorometric probe.

Bilirubin Assay Kit

MET-5010 200 assays
EUR 479
Description: Bilirubin, a byproduct of heme breakdown, can exist conjugated to glucuronic acid (direct) and as unconjugated (indirect). The unconjugated form is found in the blood bound to albumin and is transported to the liver. Bilirubin becomes conjugated to glucuronic acid in the liver, making it more soluble and allowing for excretion into bile. High levels of bilirubin have been correlated with jaundice and Gilbert?s syndrome while low levels have been associated with cardiovascular disease and diabetes mellitus.

Pyruvate Assay Kit

MET-5029 100 assays
EUR 479
Description: Our Pyruvate Assay Kit measures pyruvate in biological samples. First, pyruvate is oxidized by pyruvate oxidase, producing hydrogen peroxide. The hydrogen peroxide is then detected at ex. 530-570 nm/em. 590-600 nm using a specific fluorometric probe. Pyruvate levels in unknown samples are determined based on the provided pyruvate standard curve.

Glycine Assay Kit

MET-5070 100 assays
EUR 450

Taurine Assay Kit

MET-5071 200 assays
EUR 508

Sarcosine Assay Kit

MET-5072 100 assays
EUR 450

Tyrosine Assay Kit

MET-5073 100 assays
EUR 450

Phospholipid Assay Kit

MET-5085 96 assays
EUR 456

Ammonia Assay Kit

MET-5086 100 assays
EUR 456

Adenosine Assay Kit

MET-5090 100 assays
EUR 508

Inosine Assay Kit

MET-5092 100 assays
EUR 508

Alanine Assay Kit

MET-5093 200 assays
EUR 508

Urea Assay Kit

STA-382 192 assays
EUR 635
Description: Cell Biolabs? Urea Assay Kit is based on the Berthelot reaction.  Urea is first degraded into ammonia and carbon dioxide, which further reacts with an alkaline developer to produce a blue-green colored product that can be measured with a standard spectrophotometric plate reader at an optical density between 580-630 nm.  Each kit provides sufficient reagents to perform up to 192 assays, including blanks, urea standards and unknown samples.

Rac1 Activation Assay

STA-401-1 20 assays
EUR 757
Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit.

Rac2 Activation Assay

STA-401-2 20 assays
EUR 757
Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit.

Cdc42 Activation Assay

STA-402 20 assays
EUR 757
Description: Our Cdc42 Activation Assays use visible agarose beads to selectively precipitate the active form of Cdc42 protein. The precipitated small GTPase is then detected by Western blot using a Cdc42-specific antibody included in the kit.

RhoA Activation Assay

STA-403-A 20 assays
EUR 757
Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

RhoB Activation Assay

STA-403-B 20 assays
EUR 757
Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

RhoC Activation Assay

STA-403-C 20 assays
EUR 757
Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

Arf1 Activation Assay

STA-407-1 20 assays
EUR 757
Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit.

Arf6 Activation Assay

STA-407-6 20 assays
EUR 757
Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit.

Ral Activation Assay

STA-408 20 assays
EUR 757
Description: Our Ral Activation Assay uses visible agarose beads to selectively precipitate the active form of Ral protein. The precipitated small GTPase is then detected by Western blot using a Ral-specific antibody included in the kit.

Ran Activation Assay

STA-409 20 assays
EUR 757
Description: Our Ran Activation Assay uses visible agarose beads to selectively precipitate the active form of Ran protein. The precipitated small GTPase is then detected by Western blot using a Ran-specific antibody included in the kit.

Phosphatidylcholine Assay Kit

STA-600 96 assays
EUR 519
Description: Cell Biolabs? Phosphatidylcholine Assay Kit measures the phosphatidylcholine present within serum, plasma, or tissue samples.Samples are compared to a known concentration of phosphatidylcholine standard within the 96-well microtiter plate format.  Samples and standards are incubated for 60 minutes and then read with a standard 96-well fluorometric plate reader.

Sphingomyelin Assay Kit

STA-601 96 assays
EUR 519
Description: Cell Biolabs? Sphingomyelin Assay Kit is a simple fluorometric assay that measures the amount of sphingomyelin present in plasma or serum, tissue homogenates, or cell suspensionsin a 96-well microtiter plate format.  Each kit provides sufficient reagents to perform up to 96 assays, including blanks, sphingomyelin standards and unknown samples.  Sample sphingomyelin concentrations are determined by comparison with a known sphingomyelin standard. 
Neutrophils cross the endothelial layer extra effectively than T-cells, however upon coming into the matrix, neutrophils show excessive velocity however low persistence, whereas T-cells migrate with low velocity and quite linear migration. In conclusion, 3D imaging in real-time of leukocyte extravasation in a vessel-on-a-chip permits detailed qualitative and quantitative evaluation of various levels of the total leukocyte extravasation course of in a single assay.

LEAVE A REPLY

Please enter your comment!
Please enter your name here