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Translating the Tuberculosis Research Agenda: Much Accomplished, but Much More to Be Done.

Translating the Tuberculosis Research Agenda: Much Accomplished, but Much More to Be Done.

February 3, 2021
Diagnosis of intra-thoracic tuberculosis (ITTB) in children is difficult due to the paucibacillary nature of the disease, the challenge in collecting appropriate specimens, and the low sensitivity of smear microscopy and culture. Culture and Xpert MTB/RIF provide higher diagnostic yield in presumptive TB in adults than in children. Current study was designed to understand poor yield of diagnostic assays in children. Children with presumptive ITTB were subjected to gastric aspirates and induced sputum twice. Samples were tested by Ziehl-Neelsen stain, Xpert MTB/RIF-assay, and MGIT-960 culture. Subjects were grouped as Confirmed, Unconfirmed, and Unlikely TB, and classified as progressive primary disease (PPD, lung parenchymal lesion), and primary pulmonary complex (PPC, hilar lymphadenopathy) on chest X-ray. Of children with culture-positive TB 51/394 (12.9%), culture-negative TB 305 (77.4%), and unlikely TB 38 (9.6%), 9 (2.3%) were smear positive, while 95 (24.1%) were Xpert-MTB/RIF positive. Xpert-MTB/RIF detected 40/51 culture confirmed cases (sensitivity 78.4% and NPV 96.3%). Culture was positive in more children presenting as PPD (p < 0.04). In culture-negative TB group, Xpert positivity was seen in 31% of those with PPD and 11.9% in those with PPC (p < 0.001). Conclusion: Xpert-MTB/RIF improved diagnosis by 2-fold and increased detection of MDR-TB. Both liquid culture and Xpert-MTB/RIF gave higher yield in children with lung parenchymal lesions. Children with hilar lymphadenopathy without active lung parenchymal lesions had poor diagnostic yield even with sensitive nucleic acid amplification tests, due to paucibacillary/localized disease, suggesting possible utility of invasively collected samples in early diagnosis and treatment.

Diagnosis of intra-thoracic tuberculosis (ITTB) in children is difficult due to the paucibacillary nature of the disease, the challenge in collecting appropriate specimens, and...

September 28, 2021
slco1a1, Time Course of Renal Transcriptomics after Subchronic Exposure to Ochratoxin A in Fisher Rats
Biology Cells

Time Course of Renal Transcriptomics after Subchronic Exposure to Ochratoxin A in Fisher Rats

November 16, 2021
Optimized protocol for high-titer lentivirus production and transduction of primary fibroblasts
Biology Cells

Optimized protocol for high-titer lentivirus production and transduction of primary fibroblasts

October 5, 2021
Development of Human Toxo IgG ELISA Kit, and False-Positivity of Latex Agglutination Test for the Diagnosis of Toxoplasmosis
Biology Cells

Development of Human Toxo IgG ELISA Kit, and False-Positivity of Latex Agglutination Test for the...

October 5, 2021
Multifocal tuberculosis simulating multimetastatic colon cancer in an immunocompetent black African patient: a case report
Biology Cells

Inhalable nanoparticles delivery targeting alveolar macrophages for the treatment of pulmonary tuberculosis

September 25, 2021
Real-Time Spatial and Temporal Analysis of the Translocation of the Apoptosis-Inducing Factor in Cells
Biology Cells

Real-Time Spatial and Temporal Analysis of the Translocation of the Apoptosis-Inducing Factor in Cells

November 16, 2021
Translating the Tuberculosis Research Agenda: Much Accomplished, but Much More to Be Done.
Biology Cells

Translating the Tuberculosis Research Agenda: Much Accomplished, but Much More to Be Done.

February 3, 2021
western hybridization, Visualization of RNA Transcripts in Western Corn Rootworm (Diabrotica virgifera virgifera) and Plants by In Situ Hybridization
Biology Cells

Visualization of RNA Transcripts in Western Corn Rootworm (Diabrotica virgifera virgifera) and Plants by In...

November 16, 2021
Hsp70 exerts oncogenic activity in the Apc mutant Min mouse model.
Biology Cells

Hsp70 exerts oncogenic activity in the Apc mutant Min mouse model.

October 5, 2021
Corning Spin-X Filter Tube Filter
Biology Cells

Corning Spin-X Filter Tube Filter 25 Spin Tube Filter Pack

December 21, 2021
Crystal Structure of Mycobacterium tuberculosis Elongation Factor G1
Biology Cells

Crystal Structure of Mycobacterium tuberculosis Elongation Factor G1

September 25, 2021
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  • Application of Metabolism Assay Kits in Drug Screening and Toxicology Studies
  • Implementing a Decentralized Quality Control Strategy for Abbott ID NOW™ Influenza Testing in Point-of-Care (POC) Settings
  • The Importance of PCR Qualitative Positive Controls in Diagnostic Assays: Ensuring Reliable Results
  • Optimizing Giemsa Stain PR80 for Accurate Cytogenetic Analysis in Cancer Diagnostics
  • Standardization and Optimization of oxLDL-R1 ELISA for Cardiovascular Disease Biomarker Detection
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Tuberculosis presenting as metastatic lung cancer

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magnetic beads antibody, Analytical methods of antibody surface coverage and orientation on bio-functionalized magnetic beads: application to immunocapture of TNF-α

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Optimized protocol for high-titer lentivirus production and transduction of primary fibroblasts

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