Product Details

Cat # +Size K353-100
Size 100 assays
Kit Summary BioVision’s Maltose Phosphorylase Activity Assay Kit is the first commercial assay to determine the Maltose Phosphorylase activity in different bacterial lysates. The assay converts maltose to produce Glucose, which is then detected by a set of enzymatic reactions to generate a fluorescent product with a Ex/Em 535/587 nm. The fluorescence signal is directly proportional to the Maltose Phosphorylase activity. BioVision’s Matlose Phosphorylase Activity Assay Kit is rapid, sensitive and a convenient tool for detecting Maltose Phosphorylase activity. It can detect as low as 0.5 mU under the assay conditions.
Detection Method Fluorescence (Ex/Em 535/587 nm)
Species Reactivity Bacterial lysates
Applications • Analysis of Maltose Phosphorylase kinetics in inhibition or activation screening
• Measurement of Maltose Phosphorylase activity in different bacteria
Features & Benefits • Sensitive
• Rapid
• Convenient tool for detecting Maltose Phosphorylase activity
• It can detect as low as 0.5 mU under the assay conditions
Kit Components • Maltose Assay Buffer
• Maltose Developer
• Maltose
• Maltose Probe
• Glucose Standard
• Maltose Phosphorylase
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.
MULTIPLEX KIT PCR MASTITIS PCR kit
PCR-MPX218-48D
MULTIPLEX KIT PCR MASTITIS PCR kit
PCR-MPX218-96D
PCR Mix
L5051100

Details

Maltose phosphorylase (EC 2.4.1.8) belongs to the family of hexosyltransferases. It catalyzes maltose and inorganic phosphate to produce glucose and glucose-1-phosphate (G1P) during Maltose metabolism. Maltose phosphorylase is commonly identified in gram positive and gram negative bacteria including E. coli, Bacillus subtilis, Enterococcus sp. and Lactobacillus brevis etc. BioVision’s Maltose Phosphorylase Activity Assay Kit is the first commercial assay to determine the Maltose Phosphorylase activity in different bacterial lysates. The assay converts maltose to produce Glucose, which is then detected by a set of enzymatic reactions to generate a fluorescent product with a Ex/Em 535/587 nm. The fluorescence signal is directly proportional to the Maltose Phosphorylase activity. BioVision’s Matlose Phosphorylase Activity Assay Kit is rapid, sensitive and a convenient tool for detecting Maltose Phosphorylase activity. It can detect as low as 0.5 mU under the assay conditions.
Go Here –  store.genprice.com/  
Introduction:
Maltose phosphorylase (EC 2.4.1.8) belongs to the family of hexosyltransferases. It catalyzes maltose and inorganic phosphate to produce glucose and glucose-1-phosphate (G1P) during Maltose metabolism. Maltose phosphorylase is commonly identified in gram
positive and gram negative bacteria including E. coli, Bacillus subtilis, Enterococcus sp. and Lactobacillus brevis etc. BioVision’s Maltose Phosphorylase Activity Assay Kit is the first commercial assay to determine the Maltose Phosphorylase activity in different bacterial
lysates. The assay converts maltose to produce Glucose, which is then detected by a set of enzymatic reactions to generate a fluorescent product with a Ex/Em 535/587 nm. The fluorescence signal is directly proportional to the Maltose Phosphorylase activity. BioVision’s
Matlose Phosphorylase Activity Assay Kit is rapid, sensitive and a convenient tool for detecting Maltose Phosphorylase activity. It can detect as low as 0.5 mU under the assay conditions.
Maltose Phosphorylase Detection Solution Maltose + Pi Glucose Fluorescent Product (Ex/Em = 535/587 nm)
II. Application:
• Measurement of Maltose Phosphorylase activity in different bacteria
• Analysis of Maltose Phosphorylase kinetics in inhibition or activation screening.
Maltose Phosphorylase Activity Kit
Maltose Phosphorylase Activity Kit

Product name

Maltose Phosphorylase Activity Kit (Fluorometric)

Detection method

Colorimetric/Fluorometric

Sample type

Cell Lysate

Assay type

Enzyme activity (quantitative)

Assay duration

Multiple steps standard assay
Paraffin Wax Dispenser
HIS7000
Paraffin wax, granular (56 - 60)
GL4115-1KG
Paraffin wax, granular (56 - 60)
GL4115-5KG
8KG Histoplast PE Paraffin
HIS3324
Paraffin oil, BP, Ph. Eur. grade
GL4141-1L

Product overview

Maltose Phosphorylase Assay Kit (Fluorometric) (ab273317) is the first commercial assay to determine the Maltose Phosphorylase activity in different bacterial lysates. The assay converts maltose to produce Glucose, which is then detected by a set of enzymatic reactions to generate a fluorescent product with a Ex/Em 535/587 nm. The fluorescence signal is directly proportional to the Maltose Phosphorylase activity.

MULTIPLEX KIT PCR MASTITIS PCR kit
PCR-MPX218-48D Bioingentech
MULTIPLEX KIT PCR MASTITIS PCR kit
PCR-MPX218-96D Bioingentech
MULTIPLEX KIT PCR Babesia & Theileria PCR kit
PCR-MPX401-48D Bioingentech
MULTIPLEX KIT PCR Babesia & Theileria PCR kit
PCR-MPX401-96D Bioingentech
Blood PCR Kit
20-abx09801320ulSystems Abbexa

This Kit is rapid, sensitive and a convenient tool for detecting Maltose Phosphorylase activity. It can detect as low as 0.5 mU under the assay conditions.

Notes

 

Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Storage Conditions and Reagent Preparation:
Store the kit at -20°C. The kit components are stable for one year when stored as recommended. Briefly centrifuge small vials at low speed prior to opening. Read the entire protocol before performing the experiment.
 Maltose Assay Buffer: Ready to use as supplied. Warm bottle to room temperature (RT) before use. Store at 4°C.
 Maltose Probe (in DMSO): Ready to use as supplied. Warm to RT prior to use to melt frozen DMSO. Store at -20°C, protected from
light and moisture. Use within two months.
 Maltose Developer and Maltose: Reconstitute each component with 220 µl of Assay Buffer. Store at -20°C. Keep on ice while in use.
Use within two months.
 Glucose Standard: Store at -20°C. Warm to RT before use.
 Maltose Phosphorylase: Add 450 μl of 50% glycerol (not included) to the vial to prepare the enzyme stock. Vortex to mix. Aliquot and
store at -20°C. Avoid multiple freeze-thaw cycles. Use within two months.

VII. Maltose Phosphorylase Activity Assay Protocol:

1. Sample Preparation: Grow cells in a suitable growth medium at 37°C overnight. After incubation, centrifuge at 10,000 x g for 20 min to harvest the cells and measure the weight of the pellet. Add 5 ml of ice-cold PBS into the cell pellet and disperse the pellet. Sonicate the cells for 5-10 min on ice. After sonication, centrifuge the cells at 10,000 x g for 30 min and transfer the supernatant to a new tube. For Sample and Background wells, add 2 μl of the cell supernatant to the desired well(s) in a 96-well black flat-bottom plate. Adjust the volume to 50 μl using Maltose Assay Buffer.
For Positive Control, prepare a 10 fold dilution of the Maltose Phosphorylase enzyme by adding 5 μl of the enzyme with 45 μl of Maltose Assay Buffer. Add 2 μl of the diluted enzyme into the Positive Control well. Adjust the volume to 50 μl using Maltose Assay Buffer.
2. Standard Curve Preparation: Mix 10 µl of Glucose Standard with 990 µl of Assay Buffer to prepare 1 mM diluted Glucose Standard. Mix 100 µl of 1 mM diluted Glucose Standard with 900 µl of Assay Buffer to prepare 0.1 mM diluted Glucose Standard solution. Add 0,4, 8, 12, 16 and 20 μl of the 0.1 mM diluted Standard into the desired wells to generate 0, 0.4, 0.8, 1.2, 1.6 and 2.0 nmole of Standard/well respectively. Adjust the volume to 50 μl using Maltose Assay Buffer.

Maltose
LCS-4188 Scientific Laboratory Supplies EACH 248.4 EUR
Maltose
HY-N2024 MedChemExpress 100mg 129.6 EUR
Maltose 500gm
216830 Scientific Laboratory Supplies EACH 154.8 EUR
Maltose monohydrate
HY-N2024A MedChemExpress 100mg 129.6 EUR
Maltose, monohydrate
MIB1184 Bio Basic 100g 78.79 EUR
Maltose Phosphorylase
M46630002 Bio Basic 250U 138.3 EUR
Maltose Phosphorylase
M46631002 Bio Basic 1KU 271.42 EUR
Maltose Assay Kit
55R-1460 Fitzgerald 100 assays 867.6 EUR
Maltose Assay Kit
55R-1470 Fitzgerald 100 assays 847.2 EUR
D-(+)-Maltose monohydrate
abx082034-100g Abbexa 100 g 226.8 EUR
D-(+)-Maltose monohydrate
abx082249-100g Abbexa 100 g 226.8 EUR
D-(+)-Maltose, monohydrate
MB4950 Bio Basic 100g 79.84 EUR
D-(+)-Maltose monohydrate, 95%
GC1102-1KG Glentham Life Sciences 1 kg 103.2 EUR
D-(+)-Maltose monohydrate, 95%
GC1102-500G Glentham Life Sciences 500 g 74.4 EUR
D-(+)-Maltose monohydrate, 95%
GC1102-1 Glentham Life Sciences 1 55.4 EUR
D-(+)-Maltose monohydrate, 95%
GC1102-100 Glentham Life Sciences 100 15.7 EUR
D-(+)-Maltose monohydrate, 95%
GC1102-500 Glentham Life Sciences 500 31.7 EUR
E.Coli Maltose Binding Protein
20-abx262334 Abbexa
  • 994.80 EUR
  • 393.60 EUR
  • 276.00 EUR
  • 1 mg
  • 200 ug
  • 50 ug
Maltose Dehydrogenase (r-MDH)
Y46755003 Bio Basic 50KU 764.7 EUR
Maltose Dehydrogenase (r-MDH)
Y46756003 Bio Basic 10KU 208.78 EUR
Maltose Binding Protein Antibody
48666-100ul SAB 100ul 399.6 EUR
Maltose Binding Protein Antibody
48666-50ul SAB 50ul 286.8 EUR
Maltose Binding Protein Antibody
abx022501-02mg Abbexa 0.2 mg 844.8 EUR
Maltose Binding Protein Antibody
abx023318-1mg Abbexa 1 mg 1479.6 EUR
EnzyChrom™ Maltose Assay Kit
EMLT-100 BioAssay Systems 100 369 EUR
Maltose-Binding protein Antibody
20-abx137166 Abbexa
  • 1228.80 EUR
  • 594.00 EUR
  • 811.20 EUR
  • 100 ug
  • 20 ug
  • 50 ug
Maltose-Binding protein Antibody
20-abx137495 Abbexa
  • 844.80 EUR
  • 393.60 EUR
  • 276.00 EUR
  • 100 ug
  • 20 ug
  • 5 ug
D(+) Maltose in Water Std 1g/L
REMAL0101 Scientific Laboratory Supplies 100ML 187.2 EUR
Maltose Binding Protein (MBP) Protein
20-abx655552 Abbexa
  • 493.20 EUR
  • 260.40 EUR
  • 1262.40 EUR
  • 560.40 EUR
  • 360.00 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
D-(+)-Maltose monohydrate, USP/NF grade
GC5556-100G Glentham Life Sciences 100 g 52.8 EUR
D-(+)-Maltose monohydrate, USP/NF grade
GC5556-10KG Glentham Life Sciences 10 kg 457.2 EUR
D-(+)-Maltose monohydrate, USP/NF grade
GC5556-1KG Glentham Life Sciences 1 kg 103.2 EUR
D-(+)-Maltose monohydrate, USP/NF grade
GC5556-250G Glentham Life Sciences 250 g 64.8 EUR
D-(+)-Maltose monohydrate, USP/NF grade
GC5556-25KG Glentham Life Sciences 25 kg 878.4 EUR
D-(+)-Maltose monohydrate, USP/NF grade
GC5556-5KG Glentham Life Sciences 5 kg 294 EUR
D-(+)-Maltose monohydrate, USP/NF grade
GC5556-1 Glentham Life Sciences 1 55.4 EUR
D-(+)-Maltose monohydrate, USP/NF grade
GC5556-10 Glentham Life Sciences 10 348.1 EUR
D-(+)-Maltose monohydrate, USP/NF grade
GC5556-100 Glentham Life Sciences 100 15.7 EUR
D-(+)-Maltose monohydrate, USP/NF grade
GC5556-25 Glentham Life Sciences 25 696 EUR
D-(+)-Maltose monohydrate, USP/NF grade
GC5556-250 Glentham Life Sciences 250 23.8 EUR
D-(+)-Maltose monohydrate, USP/NF grade
GC5556-5 Glentham Life Sciences 5 213.7 EUR
Recombinant E.Coli Maltose Binding Protein
7-05572 CHI Scientific 5µg Ask for price
Recombinant E.Coli Maltose Binding Protein
7-05573 CHI Scientific 200µg Ask for price
Recombinant E.Coli Maltose Binding Protein
7-05574 CHI Scientific 1mg Ask for price
Maltose Binding Protein Conjugated Antibody
C48666 SAB 100ul 476.4 EUR
Maltose Colorimetric/Fluorometric Assay Kit
K628-100 Biovision each 614.4 EUR
Maltose Colorimetric/Fluorometric Assay Kit
K2132-100 ApexBio 100 assays 602.4 EUR
Maltose Phosphorylase Activity Kit (Fluorometric)
K353-100 Biovision each 639.6 EUR
Maltose Binding Protein Antibody / malE / MBP tag
RQ5298 NSJ Bioreagents 100ul 356.15 EUR
Maltose Binding Protein Antibody / malE / MBP tag
RQ5299 NSJ Bioreagents 100ul 356.15 EUR

BSA

31C-CH0114 Fitzgerald 50 mg 159.6 EUR

Testosterone 3 BSA Protein (BSA)

abx060856-1mg Abbexa 1 mg 693.6 EUR

BSA Control for Age-BSA

2221-BSA Biovision each 210 EUR

BSA Control for AGE-BSA

35R-AA007 Fitzgerald 10 mg 268.8 EUR

THC (BSA)

abx080017-1mg Abbexa 1 mg 510 EUR

SAH (BSA)

abx083009-50ug Abbexa 50 ug 460.8 EUR

PCP [BSA]

DAG3015 Creative Diagnostics 1 mg 858 EUR

THC [BSA]

DAG406 Creative Diagnostics 1mg 858 EUR

Pb2+ [BSA]

DAGA-005B Creative Diagnostics 1mg 1326 EUR

Cd2+ [BSA]

DAGA-006B Creative Diagnostics 1mg 1076.4 EUR

Hg2+ [BSA]

DAGA-007B Creative Diagnostics 1mg 1076.4 EUR

Cu2+ [BSA]

DAGA-008B Creative Diagnostics 1mg 936 EUR

MDMA (BSA)

abx080012-1mg Abbexa 1 mg 444 EUR

EDDP (BSA)

abx080038-1mg Abbexa 1 mg 844.8 EUR

DHEA [BSA]

DAGPY6646 Creative Diagnostics 500ug 1014 EUR

gABA (BSA)

20-abx165727 Abbexa
  • 693.60 EUR
  • 309.60 EUR
  • 2064.00 EUR
  • 828.00 EUR
  • 510.00 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Opium (BSA)

abx080050-1mg Abbexa 1 mg 543.6 EUR

BSA-SAH

ACT00301-50 Arthus 50µg (20µg) 289.2 EUR

BSA-HCY

ACT00501-100 Arthus 100µg 487.2 EUR

Sudan [BSA]

DAGA-075B Creative Diagnostics 1mg 1014 EUR

BSA-SAH

E62K00201 EnoGene 100ug 411.6 EUR

Biotin [BSA]

DAG3197 Creative Diagnostics 1mg 577.2 EUR

Cocaine (BSA)

abx080052-1mg Abbexa 1 mg 543.6 EUR

Digoxin [BSA]

DAG3043 Creative Diagnostics 0.5ml 858 EUR

Glycine [BSA]

DAG3313 Creative Diagnostics 250ug 858 EUR

Glyoxal [BSA]

DAG3314 Creative Diagnostics 250ug 936 EUR

Tylosin [BSA]

DAGA-047B Creative Diagnostics 1mg 1014 EUR

Patulin [BSA]

DAGA-368B Creative Diagnostics 1mg 1076.4 EUR

Carmine (BSA)

20-abx165660 Abbexa
  • 693.60 EUR
  • 309.60 EUR
  • 2013.60 EUR
  • 811.20 EUR
  • 510.00 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Estriol (BSA)

20-abx165717 Abbexa
  • 693.60 EUR
  • 309.60 EUR
  • 2064.00 EUR
  • 828.00 EUR
  • 510.00 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Glycine (BSA)

20-abx165740 Abbexa
  • 727.20 EUR
  • 309.60 EUR
  • 2180.40 EUR
  • 861.60 EUR
  • 526.80 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Heparin (BSA)

20-abx165746 Abbexa
  • 661.20 EUR
  • 292.80 EUR
  • 1914.00 EUR
  • 777.60 EUR
  • 493.20 EUR
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

BSA Antigen

IM112 Cygnus Technologies 1 ml 342 EUR

BSA antibody

10-1911 Fitzgerald 200 ul 651.6 EUR

BSA antibody

10R-10573 Fitzgerald 100 ul 418.8 EUR

BSA antibody

10R-1795 Fitzgerald 100 ul 522 EUR

BSA antibody

10R-A139B Fitzgerald 500 µg 925.2 EUR

BSA antibody

20R-AR042 Fitzgerald 20 mg 392.4 EUR

BSA antibody

20R-1871 Fitzgerald 100 ug 807.6 EUR

BSA antibody

70R-AS001 Fitzgerald 500 ug 236.4 EUR

Ketamine (BSA)

abx080008-1mg Abbexa 1 mg 1345.2 EUR

Morphine (BSA)

abx080032-1mg Abbexa 1 mg 444 EUR

Morphine (BSA)

abx080033-1mg Abbexa 1 mg 444 EUR

Morphine (BSA)

abx080034-1mg Abbexa 1 mg 444 EUR

Ketamine (BSA)

abx080046-1mg Abbexa 1 mg 543.6 EUR

Ketamine (BSA)

abx080047-1mg Abbexa 1 mg 627.6 EUR

Barbital (BSA)

abx080049-1mg Abbexa 1 mg 543.6 EUR

Morphine (BSA)

abx080051-1mg Abbexa 1 mg 543.6 EUR

Cotinine (BSA)

abx080054-1mg Abbexa 1 mg 577.2 EUR

BSA Antibody

20-abx322792 Abbexa
  • 376.80 EUR
  • 292.80 EUR
  • 100 ug
  • 50 ug

BSA antibody

70-BC67 Fitzgerald 1 mg 229.2 EUR

BSA antibody

70C-CR1016GAP Fitzgerald 1 mg 516 EUR

BSA Antibody

5998-100 Biovision each 424.8 EUR

BSA Antibody

5998-30T Biovision each 175.2 EUR

BSA antibody

70R-12462 Fitzgerald 100 ug 552 EUR

BSA Antibody

35643-100ul SAB 100ul 302.4 EUR

BSA Antibody

48061-100ul SAB 100ul 399.6 EUR

BSA Antibody

48061-50ul SAB 50ul 286.8 EUR

Oxazepam [BSA]

DAG3106 Creative Diagnostics 1 mg 1076.4 EUR

Acrolein [BSA]

DAG3266 Creative Diagnostics 100ug 1248 EUR

Agmatine [BSA]

DAG3267 Creative Diagnostics 100ug 1014 EUR

Dopamine [BSA]

DAG3302 Creative Diagnostics 1mg 1326 EUR

Naproxen [BSA]

DAG3370 Creative Diagnostics 250ug 702 EUR

Rotenone [BSA]

DAG3400 Creative Diagnostics mg 936 EUR

Fentanyl [BSA]

DAG398 Creative Diagnostics 1 mg 836.4 EUR

Tramadol [BSA]

DAG399 Creative Diagnostics 1mg 1466.4 EUR

Ketamine [BSA]

DAG400 Creative Diagnostics 1mg 858 EUR

Cotinine [BSA]

DAG401 Creative Diagnostics 1mg 936 EUR

Neomycin [BSA]

DAG4470 Creative Diagnostics 0.5ml 936 EUR

Melamine [BSA]

DAG4476 Creative Diagnostics 1mg 936 EUR

Monensin [BSA]

DAGA-050B Creative Diagnostics 1mg 1014 EUR

Carbaryl [BSA]

DAGA-080B Creative Diagnostics 1mg 936 EUR

Diazepam [BSA]

DAGA-093B Creative Diagnostics 1mg 936 EUR

Imazalil [BSA]

DAGA-099B Creative Diagnostics 1mg 998.4 EUR

Gossypol [BSA]

DAGA-112B Creative Diagnostics 1mg 936 EUR

Citrinin [BSA]

DAGPY-0061 Creative Diagnostics 500ul 1014 EUR

BSA Antibody

1-CSB-PA272395 Cusabio
  • 380.40 EUR
  • 292.80 EUR
  • 100ul
  • 50ul
Paraffin Wax Dispenser
HIS7000
Paraffin wax, granular (56 - 60)
GL4115-1KG
Paraffin wax, granular (56 - 60)
GL4115-5KG
8KG Histoplast PE Paraffin
HIS3324
Paraffin oil, BP, Ph. Eur. grade
GL4141-1L

LEAVE A REPLY

Please enter your comment!
Please enter your name here