Glutathione S Transferase (GST) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Glutathione S Transferase (GST) concentrations in biological fluids.
Target | Glutathione S Transferase (GST) |
Reactivity | General (All species) |
Tested Applications | ELISA |
Recommended dilutions | Optimal dilutions/concentrations should be determined by the end user. |
Storage | Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit’s manual. |
Validity | The validity for this kit is 6 months. |
Stability | The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout. |
Test Range | 1.56 ng/ml – 100 ng/ml |
Sensitivity | < 0.57 ng/ml |
Standard Form | Lyophilized |
Detection Method | Colorimetric |
Assay Type | Sandwich |
Assay Data | Quantitative |
Sample Type | Biological fluids. |
Target Type | Antigen |
Assay Principle | This kit is based on Sandwich enzyme-linked immuno-sorbent assay technology. An antibody is pre-coated onto a 96-well plate. Standards, test samples, and biotin-conjugated reagent are added to the wells and incubated. A Sandwich inhibition reaction takes place between the biotin-labelled GST and the unlabelled- GST on the pre-coated antibody. The HRP-conjugated reagent is then added, and the whole plate is incubated. Unbound conjugates are removed using wash buffer at each stage. TMB substrate is used to quantify the HRP enzymatic reaction. After TMB substrate is added, only wells that contain sufficient GST will produce a blue coloured product, which then changes to yellow after adding the acidic stop solution. The intensity of the color yellow is inversely proportional to the GST amount bound on the plate. The OD is measured spectrophotometrically at 450 nm in a microplate reader, from which the concentration of GST can be calculated. |
Kit Components | The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
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Material Required But Not Provided |
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Reagent Preparation | This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
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Assay Procedure | This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
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Protocol | This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
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Results Calculation | This assay is Sandwich, therefore there is an inverse correlation between GST concentration in the sample and the absorbance measured. Create a graph with the log of the standard concentration (y-axis) and average absorbance measured (x-axis). Apply a best fit trendline through the standard points. The GST concentration of the samples can be interpolated from the standard curve. |
Assay Precision | Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Glutathione S Transferase (GST) were were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Glutathione S Transferase (GST) were tested on 3 different plates, 8 replicates in each plate. CV (%) = (Standard Deviation / mean) × 100 Intra-Assay: CV<10% Inter-Assay: CV<12% |
Availability | Shipped within 5-7 working days. |
Note | This product is for research use only.
The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments. Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein. |
Polyclonal Goat anti-GST α-form |
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GST-ANTI-1 | Detroit R&D | 50 uL | 280 EUR |
Polyclonal Goat anti-GST μ-form |
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GST-ANTI-2 | Detroit R&D | 50 uL | 280 EUR |
Polyclonal Goat anti-GST p-form |
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GST-ANTI-3 | Detroit R&D | 50 uL | 280 EUR |
Recombinant Human CSTA Protein, GST, E.coli-10ug |
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QP11547-GST-10ug | EnQuireBio | 10ug | 553 EUR |
Recombinant Human CSTA Protein, GST, E.coli-2ug |
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QP11547-GST-2ug | EnQuireBio | 2ug | 245 EUR |
Recombinant Human CSTA Protein, GST, E.coli-5ug |
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QP11547-GST-5ug | EnQuireBio | 5ug | 336 EUR |
Recombinant EBV p23 Protein, GST, E.coli-100ug |
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QP11735-GST-100ug | EnQuireBio | 100ug | 218 EUR |
Recombinant EBV p23 Protein, GST, E.coli-1mg |
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QP11735-GST-1mg | EnQuireBio | 1mg | 1061 EUR |
Recombinant EBV p23 Protein, GST, E.coli-500ug |
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QP11735-GST-500ug | EnQuireBio | 500ug | 663 EUR |
Recombinant Human ERCC1 Protein, GST, E.coli-10ug |
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QP11805-GST-10ug | EnQuireBio | 10ug | 553 EUR |
Recombinant Human ERCC1 Protein, GST, E.coli-2ug |
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QP11805-GST-2ug | EnQuireBio | 2ug | 245 EUR |
Recombinant Human ERCC1 Protein, GST, E.coli-5ug |
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QP11805-GST-5ug | EnQuireBio | 5ug | 336 EUR |
Recombinant Human PRKACA Protein, GST, E.coli-1ug |
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QP13140-GST-1ug | EnQuireBio | 1ug | 155 EUR |
Recombinant Human PRKACA Protein, GST, E.coli-50ug |
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QP13140-GST-50ug | EnQuireBio | 50ug | 1261 EUR |
Recombinant Human PRKACA Protein, GST, E.coli-5ug |
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QP13140-GST-5ug | EnQuireBio | 5ug | 201 EUR |
Recombinant Human PTEN Protein, GST, E.coli-10ug |
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QP13199-GST-10ug | EnQuireBio | 10ug | 553 EUR |
Recombinant Human PTEN Protein, GST, E.coli-2ug |
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QP13199-GST-2ug | EnQuireBio | 2ug | 245 EUR |
Recombinant Human PTEN Protein, GST, E.coli-5ug |
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QP13199-GST-5ug | EnQuireBio | 5ug | 336 EUR |
Recombinant Human RPS27A Protein, GST, E.coli-5ug |
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QP13356-GST-5ug | EnQuireBio | 5ug | 336 EUR |
Recombinant Human S100B Protein, GST, E.coli-10ug |
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QP13402-GST-10ug | EnQuireBio | 10ug | 553 EUR |
Recombinant Human S100B Protein, GST, E.coli-2ug |
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QP13402-GST-2ug | EnQuireBio | 2ug | 245 EUR |
Recombinant Human SUMO2 Protein, GST, E.coli-2ug |
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QP13649-GST-2ug | EnQuireBio | 2ug | 245 EUR |
Recombinant Human SUMO2 Protein, GST, E.coli-5ug |
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QP13649-GST-5ug | EnQuireBio | 5ug | 336 EUR |
Recombinant Human LYVE1 Protein, GST, E.coli-10ug |
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QP12609-GST-10ug | EnQuireBio | 10ug | 553 EUR |
Recombinant Human LYVE1 Protein, GST, E.coli-2ug |
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QP12609-GST-2ug | EnQuireBio | 2ug | 245 EUR |
Recombinant Human LYVE1 Protein, GST, E.coli-5ug |
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QP12609-GST-5ug | EnQuireBio | 5ug | 336 EUR |
Recombinant Human MET Protein, GST, E.coli-10ug |
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QP12683-GST-10ug | EnQuireBio | 10ug | 962 EUR |
Recombinant Human MET Protein, GST, E.coli-2ug |
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QP12683-GST-2ug | EnQuireBio | 2ug | 363 EUR |
Recombinant Human MET Protein, GST, E.coli-5ug |
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QP12683-GST-5ug | EnQuireBio | 5ug | 563 EUR |
Recombinant Human Bcl XL Protein, GST, E.coli-2ug |
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QP11141-GST-2ug | EnQuireBio | 2ug | 155 EUR |
Glutathione Transferase (GST-fusion protein) ELISA Kit, 96 tests, Quantitative |
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800-400-GST | Alpha Diagnostics | 1 kit | 712 EUR |
Recombinant other T. pallidum p15 (Partial) Protein, GST, E.coli-100ug |
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QP13670-GST-100ug | EnQuireBio | 100ug | 218 EUR |
Recombinant other T. pallidum p15 (Partial) Protein, GST, E.coli-1mg |
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QP13670-GST-1mg | EnQuireBio | 1mg | 1261 EUR |
Recombinant other T. pallidum p15 (Partial) Protein, GST, E.coli-500ug |
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QP13670-GST-500ug | EnQuireBio | 500ug | 663 EUR |
Recombinant Hepatitis C HCV NS3 Genotype-2b Protein, GST, E.coli-100ug |
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QP12167-GST-100ug | EnQuireBio | 100ug | 218 EUR |
Recombinant Hepatitis C HCV NS3 Genotype-2b Protein, GST, E.coli-1mg |
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QP12167-GST-1mg | EnQuireBio | 1mg | 1261 EUR |
Recombinant Hepatitis C HCV NS3 Genotype-2b Protein, GST, E.coli-500ug |
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QP12167-GST-500ug | EnQuireBio | 500ug | 663 EUR |
Recombinant Hepatitis C HCV NS5 Genotype-1a Protein, GST, E.coli-100ug |
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QP12187-GST-100ug | EnQuireBio | 100ug | 218 EUR |
Recombinant Hepatitis C HCV NS5 Genotype-1a Protein, GST, E.coli-1mg |
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QP12187-GST-1mg | EnQuireBio | 1mg | 1061 EUR |
Recombinant Hepatitis C HCV NS5 Genotype-1a Protein, GST, E.coli-500ug |
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QP12187-GST-500ug | EnQuireBio | 500ug | 663 EUR |
Recombinant EBV p18 Protein, GST, E.coli-100ug |
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QP11733-GST-EC-100ug | EnQuireBio | 100ug | 318 EUR |
Recombinant EBV p18 Protein, GST, E.coli-1mg |
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QP11733-GST-EC-1mg | EnQuireBio | 1mg | 1561 EUR |
Recombinant EBV p18 Protein, GST, E.coli-500ug |
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QP11733-GST-EC-500ug | EnQuireBio | 500ug | 862 EUR |
GST/ Rat GST |
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ELA-E1090r | Lifescience Market | 96 Tests | 886 EUR |
GST |
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EF003135 | Lifescience Market | 96 Tests | 689 EUR |
GST |
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EF006888 | Lifescience Market | 96 Tests | 689 EUR |
GST |
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EF006889 | Lifescience Market | 96 Tests | 689 EUR |
GST |
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EF006890 | Lifescience Market | 96 Tests | 689 EUR |
GST |
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EF006891 | Lifescience Market | 96 Tests | 689 EUR |
GST |
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EF006892 | Lifescience Market | 96 Tests | 689 EUR |
GST |
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EF006893 | Lifescience Market | 96 Tests | 689 EUR |
GST |
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EF006894 | Lifescience Market | 96 Tests | 689 EUR |
GST |
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EF012792 | Lifescience Market | 96 Tests | 689 EUR |
EBV [GST] |
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DAG1580 | Creative Diagnostics | 100 µg | 645 EUR |
EBV [GST] |
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DAG1581 | Creative Diagnostics | 100 µg | 810 EUR |
GST pAb |
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AE067 | Abclonal | 50 ul | 176 EUR |
GST Antibody |
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abx448858-100ug | Abbexa | 100 ug | 551 EUR |
GST Antibody |
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20-abx159522 | Abbexa |
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GST Antibody |
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20-abx159523 | Abbexa |
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GST Antibody |
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abx333606-100ul | Abbexa | 100 ul | 495 EUR |
GST Antibody |
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CSB-PA187265- | Cusabio | 335 EUR | |
GST Antibody |
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CSB-PA187265-100ul | Cusabio | 100ul | 379 EUR |
GST Antibody |
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48048-100ul | SAB | 100ul | 333 EUR |
GST Antibody |
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48048-50ul | SAB | 50ul | 239 EUR |
GST Antibody |
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49060-100ul | SAB | 100ul | 333 EUR |
GST Antibody |
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49060-50ul | SAB | 50ul | 239 EUR |
anti-GST |
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AR02-PA0001 | Abfrontier | 100 ul | 334 EUR |
pCold- GST |
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PVT0608 | Lifescience Market | 2 ug | 325 EUR |
pCDNA3.1- GST |
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PVT10676 | Lifescience Market | 2 ug | 301 EUR |
GST antibody |
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10R-1798 | Fitzgerald | 100 ul | 219 EUR |
GST antibody |
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10R-2934 | Fitzgerald | 100 ug | 160 EUR |
GST antibody |
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10R-3007 | Fitzgerald | 100 ug | 265 EUR |
GST antibody |
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10-2340 | Fitzgerald | 100 ug | 462 EUR |
GST antibody |
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10R-1012 | Fitzgerald | 100 ul | 316 EUR |
GST antibody |
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10R-10239 | Fitzgerald | 100 ug | 327 EUR |
GST antibody |
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10R-10442 | Fitzgerald | 100 ug | 435 EUR |
GST antibody |
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10R-10564 | Fitzgerald | 100 ug | 349 EUR |