Glutathione S Transferase (GST) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Glutathione S Transferase (GST) concentrations in biological fluids.

Target Glutathione S Transferase (GST)
Reactivity General (All species)
Tested Applications ELISA
Recommended dilutions Optimal dilutions/concentrations should be determined by the end user.
Storage Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit’s manual.
Validity The validity for this kit is 6 months.
Stability The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
Test Range 1.56 ng/ml – 100 ng/ml
Sensitivity < 0.57 ng/ml
Standard Form Lyophilized
Detection Method Colorimetric
Assay Type Sandwich
Assay Data Quantitative
Sample Type Biological fluids.
Target Type Antigen

Click Here

Assay Principle This kit is based on Sandwich enzyme-linked immuno-sorbent assay technology. An antibody is pre-coated onto a 96-well plate. Standards, test samples, and biotin-conjugated reagent are added to the wells and incubated. A Sandwich inhibition reaction takes place between the biotin-labelled GST and the unlabelled- GST on the pre-coated antibody. The HRP-conjugated reagent is then added, and the whole plate is incubated. Unbound conjugates are removed using wash buffer at each stage. TMB substrate is used to quantify the HRP enzymatic reaction. After TMB substrate is added, only wells that contain sufficient GST will produce a blue coloured product, which then changes to yellow after adding the acidic stop solution. The intensity of the color yellow is inversely proportional to the GST amount bound on the plate. The OD is measured spectrophotometrically at 450 nm in a microplate reader, from which the concentration of GST can be calculated.
Kit Components The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

  • Pre-coated 96-Well Microplate
  • Standard
  • Standard Diluent Buffer
  • Wash Buffer
  • Detection Reagent A
  • Detection Reagent B
  • Diluent A
  • Diluent B
  • TMB Substrate
  • Stop Solution
  • Plate Sealer
Material Required But Not Provided
  • 37°C incubator
  • Multi and single channel pipettes and sterile pipette tips
  • Squirt bottle or automated microplate washer
  • 1.5 ml tubes
  • Distilled water
  • Absorbent filter papers
  • 100 ml and 1 liter graduated cylinders
  • Microplate reader (wavelength: 450 nm)
  • ELISA Shaker
Reagent Preparation This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

  • 1) Standard: Prepare the standard with the recommended volume of Standard Diluent Buffer, to make the standard solution. Then use the Standard Diluent buffer to carry out serial dilutions of the standard solution, as instructed in the Protocol.
  • 2) Wash Buffer: Dilute the concentrated Wash Buffer with distilled water, as instructed in the Protocol.
  • 3) Detection Reagent Preparation: Calculate the total volume of working solution required. Dilute Detection Reagent A and Detection Reagent B with Diluent A and Diluent B, respectively, at 1:100.
Assay Procedure This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

  • 1) Set standard, test samples and control wells.
  • 2) Aliquot 50 µl of diluted standard into the standard wells.
  • 3) Aliquot 50 µl of Standard Diluent buffer into the control (zero) well.
  • 4) Aliquot 50 µl of diluted samples into the sample wells.
  • 5) Immediately aliquot 50 µl of Detection Reagent A to each well. Incubate for 1 hr at 37 °C.
  • 6) Wash 3 times.
  • 7) Aliquot 100 µl of Detection Reagent B to each well. Incubate for 30 mins at 37 °C.
  • 8) Wash 5 times.
  • 9) Aliquot 90 µl of TMB Substrate to each well. Incubate for 10-20 mins at 37 °C.
  • 10) Aliquot 50 µl of Stop Solution.
  • 11) Measure the OD at 450 nm.
Protocol This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

  • Equilibrate the kit components and samples to room temperature (18 – 25 °C) before use. It is recommended to plot a standard curve for each test.
  • 1. Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample at least in duplicate.
  • 2. Add 50 µL of each standard, control and sample into the appropriate wells.
  • 3. Remove the cover and discard the liquid.
  • 4. Immediately aliquot 50 µl of Detection Reagent A working solution. Seal the plate with a cover and incubate for 1 h at 37°C.
  • 5. Remove the cover and discard the solution. Wash the plate 3 times with 1X Wash Buffer.
  • 6. Add 100 µL of Detection Reagent B working solution into each well, seal and incubate at 37°C for 30 min.
  • 7. Discard the solution and wash the plate 5 times with wash buffer as explained in previous step.
  • 8. Aliquot 90 µl of TMB Substrate into each well. Seal the plate with a cover and incubate at 37°C for 10-20 min. Avoid exposure to light. The incubation time is for reference use only, the optimal time should be determined by end user. Do not exceed 30 min.
  • 9. Add 50 µL of Stop Solution to each well. Read at 450 nm immediately.
Results Calculation This assay is Sandwich, therefore there is an inverse correlation between GST concentration in the sample and the absorbance measured. Create a graph with the log of the standard concentration (y-axis) and average absorbance measured (x-axis). Apply a best fit trendline through the standard points. The GST concentration of the samples can be interpolated from the standard curve.
Assay Precision Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Glutathione S Transferase (GST) were were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Glutathione S Transferase (GST) were tested on 3 different plates, 8 replicates in each plate.

CV (%) = (Standard Deviation / mean) × 100

Intra-Assay: CV<10%

Inter-Assay: CV<12%

Availability Shipped within 5-7 working days.
Note This product is for research use only.

The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments.

Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.

 

anit gst gst50 nm 2 ml

gst Clemente Associates LLC 2 ML 420 EUR

GST/ Rat GST

ELA-E1090r Lifescience Market 96 Tests 1063.2 EUR

Glutathione S-Transferase, gst, GST tag (GST) Antibody

abx341009-100l Abbexa 100 µl 275 EUR

Glutathione S-Transferase, gst, GST tag (GST) Antibody

abx341009-50l Abbexa 50 µl 212.5 EUR

GST

E8EM80701 EnoGene 100ul 275 EUR

GST

EF012792 Lifescience Market 96 Tests 826.8 EUR

GST

EF003135 Lifescience Market 96 Tests 826.8 EUR

GST

EF006888 Lifescience Market 96 Tests 826.8 EUR

GST

EF006889 Lifescience Market 96 Tests 826.8 EUR

GST

EF006890 Lifescience Market 96 Tests 826.8 EUR

GST

EF006891 Lifescience Market 96 Tests 826.8 EUR

GST

EF006892 Lifescience Market 96 Tests 826.8 EUR

GST

EF006893 Lifescience Market 96 Tests 826.8 EUR

GST

EF006894 Lifescience Market 96 Tests 826.8 EUR

GST

E8ET1611-47 EnoGene 100ul 275 EUR

GST

ant-164 ProSpec Tany 0.5mg 275 EUR

GST

ant-219 ProSpec Tany 100µg 155 EUR

GST

ant-456 ProSpec Tany 5µg 60 EUR

GST

enz-393 ProSpec Tany 10µg 60 EUR

GST

GWB-BBB159 GenWay Biotech 0.01 mg Ask for price

GST

PC-252 Kamiya Biomedical Company Polyclonal Ask for price

GST

PC-253 Kamiya Biomedical Company Polyclonal Ask for price

GST

Mab-605081 Antagene 0.1mg 99 EUR

EBV [GST]

DAG1580 Creative Diagnostics 100 µg 774 EUR

EBV [GST]

DAG1581 Creative Diagnostics 100 µg 972 EUR

LIF, GST

cyt-001 ProSpec Tany 2µg 60 EUR

MIF GST

cyt-401 ProSpec Tany 5µg 60 EUR

GST HRP

ant-220 ProSpec Tany 100µl 315 EUR

IL8 GST

chm-047 ProSpec Tany 100µg 200 EUR

GST pAb

AE067 Abclonal 50 ul 211.2 EUR

GST His

enz-451 ProSpec Tany 10µg 60 EUR

CD2 GST

pro-468 ProSpec Tany 2µg 175 EUR

APOD, GST

cyt-652 ProSpec Tany 2µg 175 EUR

IGF1, GST

cyt-690 ProSpec Tany 2µg 175 EUR

MMP3 GST

enz-455 ProSpec Tany 2µg 175 EUR

mBID GST

pro-643 ProSpec Tany 2µg 60 EUR

mBAX GST

pro-647 ProSpec Tany 2µg 60 EUR

CD34 GST

pro-292 ProSpec Tany 2µg 175 EUR

FHIT, GST

pro-297 ProSpec Tany 2µg 175 EUR

KRT8 GST

pro-298 ProSpec Tany 2µg 175 EUR

GST-Pi

MC-411 Kamiya Biomedical Company Usal-hGST-Pi-McAb-1 Ask for price

GST-HRP

PC-323 Kamiya Biomedical Company Polyclonal Ask for price

GST-HRP

PC-324 Kamiya Biomedical Company Polyclonal Ask for price

pCold- GST

PVT0608 Lifescience Market 2 ug 390 EUR

sRANKL, GST

cyt-631 ProSpec Tany 2µg 60 EUR

GST-FITC

PC-321 Kamiya Biomedical Company Polyclonal Ask for price

GST-FITC

PC-322 Kamiya Biomedical Company Polyclonal Ask for price

pCDNA3.1- GST

PVT10676 Lifescience Market 2 ug 361.2 EUR

GST protein

30R-1157 Fitzgerald 100 ug 307 EUR

GST protein

30R-2415 Fitzgerald 1 mg 165 EUR

GST antibody

10-2340 Fitzgerald 100 ug 554.4 EUR

GST antibody

10R-1012 Fitzgerald 100 ul 320 EUR

GST antibody

10R-10239 Fitzgerald 100 ug 539 EUR

GST antibody

10R-10442 Fitzgerald 100 ug 451 EUR

GST antibody

10R-10564 Fitzgerald 100 ug 344 EUR

GST antibody

10R-1798 Fitzgerald 100 ul 193 EUR

GST antibody

10R-2934 Fitzgerald 100 ug 120 EUR

GST antibody

10R-3007 Fitzgerald 100 ug 218 EUR

GST antibody

10R-7762 Fitzgerald 500 ug 678 EUR

GST Antibody

48048 SAB 100ul 429 EUR

GST Antibody

48048-100ul SAB 100ul 399.6 EUR

GST Antibody

48048-50ul SAB 50ul 286.8 EUR

GST Antibody

49060 SAB 100ul 499 EUR

GST Antibody

49060-100ul SAB 100ul 399.6 EUR

GST Antibody

49060-50ul SAB 50ul 286.8 EUR

GST Antibody

abx333606-100ul Abbexa 100 ul 594 EUR

GST Antibody

20-abx159522 Abbexa
  • Ask for price
  • Ask for price
  • 100 ug
  • 50 ug

GST Antibody

20-abx159523 Abbexa
  • Ask for price
  • Ask for price
  • 100 ug
  • 50 ug

GST Antibody

abx448858-100ug Abbexa 100 ug 661.2 EUR

GST Antibody

E10-20033 EnoGene 100μg/100μl 225 EUR

GST Antibody

E81003-8 EnoGene 100ul 225 EUR

GST Antibody

E8M0807-1 EnoGene 100ul 225 EUR

GST Antibody

E8M0807-12 EnoGene 100ul 225 EUR

GST Antibody

E300003a EnoGene 100ug/200ul 295 EUR

GST antibody

70R-13723 Fitzgerald 100 ug 519 EUR

GST antibody

70R-14127 Fitzgerald 100 ug 519 EUR

GST Antibody

CSB-PA187265- Cusabio each 402 EUR

Source :

  1. NCBI
  2. Gentaur

LEAVE A REPLY

Please enter your comment!
Please enter your name here