Glutathione S Transferase (GST) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Glutathione S Transferase (GST) concentrations in biological fluids.

Target Glutathione S Transferase (GST)
Reactivity General (All species)
Tested Applications ELISA
Recommended dilutions Optimal dilutions/concentrations should be determined by the end user.
Storage Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit’s manual.
Validity The validity for this kit is 6 months.
Stability The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
Test Range 1.56 ng/ml – 100 ng/ml
Sensitivity < 0.57 ng/ml
Standard Form Lyophilized
Detection Method Colorimetric
Assay Type Sandwich
Assay Data Quantitative
Sample Type Biological fluids.
Target Type Antigen

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Assay Principle This kit is based on Sandwich enzyme-linked immuno-sorbent assay technology. An antibody is pre-coated onto a 96-well plate. Standards, test samples, and biotin-conjugated reagent are added to the wells and incubated. A Sandwich inhibition reaction takes place between the biotin-labelled GST and the unlabelled- GST on the pre-coated antibody. The HRP-conjugated reagent is then added, and the whole plate is incubated. Unbound conjugates are removed using wash buffer at each stage. TMB substrate is used to quantify the HRP enzymatic reaction. After TMB substrate is added, only wells that contain sufficient GST will produce a blue coloured product, which then changes to yellow after adding the acidic stop solution. The intensity of the color yellow is inversely proportional to the GST amount bound on the plate. The OD is measured spectrophotometrically at 450 nm in a microplate reader, from which the concentration of GST can be calculated.
Kit Components The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

  • Pre-coated 96-Well Microplate
  • Standard
  • Standard Diluent Buffer
  • Wash Buffer
  • Detection Reagent A
  • Detection Reagent B
  • Diluent A
  • Diluent B
  • TMB Substrate
  • Stop Solution
  • Plate Sealer
Material Required But Not Provided
  • 37°C incubator
  • Multi and single channel pipettes and sterile pipette tips
  • Squirt bottle or automated microplate washer
  • 1.5 ml tubes
  • Distilled water
  • Absorbent filter papers
  • 100 ml and 1 liter graduated cylinders
  • Microplate reader (wavelength: 450 nm)
  • ELISA Shaker
Reagent Preparation This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

  • 1) Standard: Prepare the standard with the recommended volume of Standard Diluent Buffer, to make the standard solution. Then use the Standard Diluent buffer to carry out serial dilutions of the standard solution, as instructed in the Protocol.
  • 2) Wash Buffer: Dilute the concentrated Wash Buffer with distilled water, as instructed in the Protocol.
  • 3) Detection Reagent Preparation: Calculate the total volume of working solution required. Dilute Detection Reagent A and Detection Reagent B with Diluent A and Diluent B, respectively, at 1:100.
Assay Procedure This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

  • 1) Set standard, test samples and control wells.
  • 2) Aliquot 50 µl of diluted standard into the standard wells.
  • 3) Aliquot 50 µl of Standard Diluent buffer into the control (zero) well.
  • 4) Aliquot 50 µl of diluted samples into the sample wells.
  • 5) Immediately aliquot 50 µl of Detection Reagent A to each well. Incubate for 1 hr at 37 °C.
  • 6) Wash 3 times.
  • 7) Aliquot 100 µl of Detection Reagent B to each well. Incubate for 30 mins at 37 °C.
  • 8) Wash 5 times.
  • 9) Aliquot 90 µl of TMB Substrate to each well. Incubate for 10-20 mins at 37 °C.
  • 10) Aliquot 50 µl of Stop Solution.
  • 11) Measure the OD at 450 nm.
Protocol This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

  • Equilibrate the kit components and samples to room temperature (18 – 25 °C) before use. It is recommended to plot a standard curve for each test.
  • 1. Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample at least in duplicate.
  • 2. Add 50 µL of each standard, control and sample into the appropriate wells.
  • 3. Remove the cover and discard the liquid.
  • 4. Immediately aliquot 50 µl of Detection Reagent A working solution. Seal the plate with a cover and incubate for 1 h at 37°C.
  • 5. Remove the cover and discard the solution. Wash the plate 3 times with 1X Wash Buffer.
  • 6. Add 100 µL of Detection Reagent B working solution into each well, seal and incubate at 37°C for 30 min.
  • 7. Discard the solution and wash the plate 5 times with wash buffer as explained in previous step.
  • 8. Aliquot 90 µl of TMB Substrate into each well. Seal the plate with a cover and incubate at 37°C for 10-20 min. Avoid exposure to light. The incubation time is for reference use only, the optimal time should be determined by end user. Do not exceed 30 min.
  • 9. Add 50 µL of Stop Solution to each well. Read at 450 nm immediately.
Results Calculation This assay is Sandwich, therefore there is an inverse correlation between GST concentration in the sample and the absorbance measured. Create a graph with the log of the standard concentration (y-axis) and average absorbance measured (x-axis). Apply a best fit trendline through the standard points. The GST concentration of the samples can be interpolated from the standard curve.
Assay Precision Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Glutathione S Transferase (GST) were were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Glutathione S Transferase (GST) were tested on 3 different plates, 8 replicates in each plate.

CV (%) = (Standard Deviation / mean) × 100

Intra-Assay: CV<10%

Inter-Assay: CV<12%

Availability Shipped within 5-7 working days.
Note This product is for research use only.

The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments.

Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.

 

GST/ Rat GST

ELA-E1090r Lifescience Market 96 Tests 1063.2 EUR

GST

EF003135 Lifescience Market 96 Tests 826.8 EUR

GST

EF006888 Lifescience Market 96 Tests 826.8 EUR

GST

EF006889 Lifescience Market 96 Tests 826.8 EUR

GST

EF006890 Lifescience Market 96 Tests 826.8 EUR

GST

EF006891 Lifescience Market 96 Tests 826.8 EUR

GST

EF006892 Lifescience Market 96 Tests 826.8 EUR

GST

EF006893 Lifescience Market 96 Tests 826.8 EUR

GST

EF006894 Lifescience Market 96 Tests 826.8 EUR

GST

EF012792 Lifescience Market 96 Tests 826.8 EUR

GST pAb

AE067 Abclonal 50 ul 211.2 EUR

EBV [GST]

DAG1580 Creative Diagnostics 100 µg 774 EUR

EBV [GST]

DAG1581 Creative Diagnostics 100 µg 972 EUR

pCold- GST

PVT0608 Lifescience Market 2 ug 390 EUR

pCDNA3.1- GST

PVT10676 Lifescience Market 2 ug 361.2 EUR

GST protein

30R-1157 Fitzgerald 100 ug 366 EUR

GST protein

30R-2415 Fitzgerald 1 mg 256.8 EUR

GST antibody

70R-13723 Fitzgerald 100 ug 386.4 EUR

GST Antibody

48048-100ul SAB 100ul 399.6 EUR

GST Antibody

48048-50ul SAB 50ul 286.8 EUR

GST Antibody

49060-100ul SAB 100ul 399.6 EUR

GST Antibody

49060-50ul SAB 50ul 286.8 EUR

GST antibody

70R-14127 Fitzgerald 100 ug 386.4 EUR

GST antibody

10-2340 Fitzgerald 100 ug 554.4 EUR

GST antibody

10R-1798 Fitzgerald 100 ul 262.8 EUR

GST antibody

10R-2934 Fitzgerald 100 ug 192 EUR

GST antibody

10R-3007 Fitzgerald 100 ug 318 EUR

GST antibody

10R-7762 Fitzgerald 500 ug 678 EUR

GST antibody

10R-1012 Fitzgerald 100 ul 379.2 EUR

GST antibody

10R-10239 Fitzgerald 100 ug 392.4 EUR

GST antibody

10R-10442 Fitzgerald 100 ug 522 EUR

GST antibody

10R-10564 Fitzgerald 100 ug 418.8 EUR

GST Antibody

abx333606-100ul Abbexa 100 ul 594 EUR

GST Antibody

20-abx159522 Abbexa
  • 326.40 EUR
  • 276.00 EUR
  • 100 ug
  • 50 ug

GST Antibody

20-abx159523 Abbexa
  • 326.40 EUR
  • 276.00 EUR
  • 100 ug
  • 50 ug

GST Antibody

abx448858-100ug Abbexa 100 ug 661.2 EUR

GST Antibody

CSB-PA187265- Cusabio each 402 EUR

GST Antibody

CSB-PA187265-100ul Cusabio 100ul 454.8 EUR

GST Antibody

F52061-0.08ML NSJ Bioreagents 0.08 ml 140.25 EUR

GST Antibody

F52061-0.4ML NSJ Bioreagents 0.4 ml 330.65 EUR

GST Antibody

F52068-0.08ML NSJ Bioreagents 0.08 ml 140.25 EUR

GST Antibody

F52068-0.4ML NSJ Bioreagents 0.4 ml 322.15 EUR

Recombinant purified human GST-mu (GST-M2) protein

GSTM35-R-25 Alpha Diagnostics 25 ug 416.4 EUR

Alpha-GST/ Rat Alpha- GST ELISA Kit

ELA-E0609r Lifescience Market 96 Tests 1063.2 EUR

GST pi Antibody (GST3)

R30217 NSJ Bioreagents 100 ug 356.15 EUR

RB, GST-tag

40595 BPS Bioscience 200 µg 455 EUR

EV71 VP0 [GST]

DAG-H10313 Creative Diagnostics 10µg 868.8 EUR

EV71 VP1 [GST]

DAG1665 Creative Diagnostics 2 µg 1060.8 EUR

CMV UL55 [GST]

DAG1842 Creative Diagnostics 500 ug 3034.8 EUR

CMV Pp65 [GST]

DAG1847 Creative Diagnostics 500 ug 3034.8 EUR

GST (aa 1 - 242)

DAG1663 Creative Diagnostics 10 ug 1092 EUR

Gst Spin Trap

28952359 Scientific Laboratory Supplies PK50 516 EUR

LTK, GST-Tag

101357 BPS Bioscience 10 µg 410 EUR

CBL, GST-Tag

100370 BPS Bioscience 100 µg 345 EUR

FXR, GST-Tag

100410 BPS Bioscience 50 µg 455 EUR

TTK, GST-tag

40291 BPS Bioscience 10 µg 410 EUR

RON, GST-tag

40292 BPS Bioscience 10 µg 410 EUR

ZAK, GST-tag

40297 BPS Bioscience 10 µg 410 EUR

BLK, GST-tag

40401 BPS Bioscience 10 µg 410 EUR

BMX, GST-tag

40402 BPS Bioscience 10 µg 355 EUR

BRK, GST-tag

40403 BPS Bioscience 10 µg 355 EUR

BTK, GST-tag

40405 BPS Bioscience 10 µg 355 EUR

CSK, GST-tag

40410 BPS Bioscience 10 µg 355 EUR

FAK, GST-tag

40420 BPS Bioscience 10 µg 355 EUR

FGR, GST-tag

40422 BPS Bioscience 10 µg 410 EUR

FRK, GST-tag

40424 BPS Bioscience 10 µg 410 EUR

HCK, GST-tag

40440 BPS Bioscience 10 µg 410 EUR

ITK, GST-tag

40445 BPS Bioscience 10 µg 355 EUR

LCK, GST-tag

40470 BPS Bioscience 10 µg 365 EUR

SRC, GST-tag

40483 BPS Bioscience 10 µg 355 EUR

TXK, GST-tag

40486 BPS Bioscience 10 µg 410 EUR

p53, GST-tag

40511 BPS Bioscience 20 µg 325 EUR

ICK, GST-tag

40112 BPS Bioscience 10 µg 410 EUR

NDR, GST-tag

40138 BPS Bioscience 10 µg 410 EUR

Axl, GST-tag

40180 BPS Bioscience 10 µg 355 EUR

ALK , GST-tag

40214 BPS Bioscience 10 µg 355 EUR

IRR, GST-tag

40242 BPS Bioscience 10 µg 410 EUR

Source :

  1. NCBI
  2. Gentaur

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