B cells are responsible for producing and releasing antibodies to specific antigens, and hence, are an essential component of the humoral immune response.
B cells are mediators of the humoral response, or antibody-mediated immunity. By studying this particular cell group we learn more about the inner workings of the immune system, which consequently increases our awareness of the possible causes behind a variety of autoimmune disorders and cancers. Broad immunological research unlocks valuable insight of what future steps might be taken to treat these pathologies.
Development from stem cell to B cell
Generation of the B cell begins in the bone marrow where stem cells give rise to lymphoid cells. Throughout each stage of development the antibody locus— a site where an antigen interacts with the cell— undergoes genetic recombination. This recombination is specific to the developmental stage of the B cell. Development starts with the pro-B cell, which expresses Igα and Igβ. The cell matures further into the pre-B cell that expresses the pre-B cell receptor (Igμ) on its surface. Maturation in the bone marrow ends with the naïve B cell that expresses the B cell receptor (containing IgM and IgD) capable of recognizing an antigen. These cells then leave the bone marrow and enter the periphery (Cambier JC, et al. Nat Rev Immunol. 2007).
Subtypes of conventional B cells
Conventional B cells, also referred to as B-2 cells, terminally differentiate into one of two common subtypes upon activation:
- Plasma B cells: a plasma cell is the sentry of the immune system. The naïve B cell circulates throughout the body. When it encounters a unique antigen, the plasma cell takes in the antigen through receptor-mediated endocytosis. Antigenic particles are transferred to the cell surface, loaded onto MHC II molecules and presented to a helper T cell. The binding of the helper T cell to the MHC II-antigen complex activates the B cell. The activated B cell goes through a period of rapid proliferation and somatic hypermutation. Selection occurs for those cells that produce antibodies with a high affinity for that particular antigen. Once terminally differentiated, the plasma B cell only secretes antibodies specific for that antigen and can no longer generate antibodies to other antigens.
- Memory B cells: memory cells are held in reserve, in the germinal centers of the lymphatic system, for when the immune system re-encounters a specific antigen. During any repeat exposure the follicular helper T cell causes the memory cell to differentiate into a plasma B cell that has a greater sensitivity to that specific antigen. This jump-starts the immune system to mount a quicker, more powerful response than was possible previously.
Other B cell subtypes include:
- B-1 cells: a minor subtype, only about 5% in humans, of self-renewing fetal B cells that act in a similar fashion to plasma cells. B-1 cells are primarily present during fetal and neonatal life.
- Marginal zone (MZ) B cells: mature memory B cells that are found only in the marginal zone of the spleen. These cells can be activated through toll-like receptor-ligation and not necessarily through the B cell receptor.
- Follicular (FO) B cells: these are mature, but inactive, B cells. This subset of B cells is primarily found in the follicles of the spleen and lymph nodes. Activation of these cells requires the aid of T cells. FO B cells can differentiate into either plasma or memory B cells.
- Regulatory B (Breg) cells: Breg cells negatively regulate the strength of the immune response and inflammation by secreting chemical messages called cytokines, such as IL-10. Although these cells make up a small portion of the B cell population (~0.5% in humans), it is thought that loss of functional Breg cells contributes to autoimmune disorders.
Paraffin Wax Dispenser | |||
HIS7000 | |||
Paraffin wax, granular (56 - 60) | |||
GL4115-1KG | |||
Paraffin wax, granular (56 - 60) | |||
GL4115-5KG | |||
Paraffin wax, granular (56 - 60) | |||
GL4115-1 | |||
Paraffin wax, granular (56 - 60) | |||
GL4115-5 |
Immunophenotyping of B cells through flow cytometry
Immature B cells express CD19, CD 20, CD34, CD38, and CD45R, but not IgM. For most mature B cells the key markers include IgM and CD19, a protein receptor for antigens (Kaminski DA. Front Immunol. 2012). Activated B cells express CD30, a regulator of apoptosis. Plasma B cells lose CD19 expression, but gain CD78, which is used to quantify these cells. Memory B cells can be immunophenotyped using CD20 and CD40 expression. The cells can be further categorized using CD80 and PDL-2 regardless of the type of immunoglobulin present on the cell surface (Zuccarino-Catania GV et al. Nat Immunol. 2014.). Globally, cytokines (such as interlukein-10) and chemokines involved with chemokine receptor 3 play an important role in transmitting the biological messages to drive the immune response.

A table of common B cell subtypes with some cell markers which can be useful for flow cytometry:
B Cell Type | Marker | Cellular localization |
B cell (all except plasma cell) | IgM, CD19 | Secreted |
Activated B cell | CD19, CD25, CD30 | Secreted |
Plasma cell | IgG, CD27, CD38, CD78, CD138, CD319 | Secreted |
Plasma cell | IL-6 | Secreted (cytokine) |
Plasma cell | CD138 | Cell membrane |
Memory cell | IgA, IgG, IgE, CD20, CD27, CD40, CD80, PDL-2 | Secreted |
Memory cell | CXCR3, CXCR4, CXCR5, CXCR6 | Secreted (chemokines) |
Marginal zone B cells | CD1, CD21, CD27 | Secreted |
Marginal zone B cells | Notch2 | Cell membrane |
Follicular B cells | IgD, CD21, CD22, CD23 | Secreted |
Regulatory B cells | IgD, CD1, CD5, CD21, CD24, TLR4 | Secreted |
Regulatory B cells | IL-10, TGFβ | Secreted (cytokines) |
The immune system consists of many different cell types, whose functions and interactions govern the immune response. In research, the specific composition of cell surface antigens is exploited to accurately define the different cell types. Immunophenotyping by flow cytometry is the most common method to identify, quantify, and isolate immune cells within mixed populations. However, selecting which antigens are best to identify a specific cell type can be challenging due to the vast number of research articles and lack of consensus around immunophenotyping panels.
In response to this issue, we have reviewed over 250 research articles studying human and mouse cells and compiled the most commonly used markers for each immune cell type. With this selection, you can quickly and easily build your own immunophenotyping panel, freeing up more of your time for research.
Immune cell markers
Many immune cell markers are named following the clusters of differentiation (CD) nomenclature, aimed at providing targets for cell immunophenotyping. In the table below we have summarized the relevant CD markers for the identification and quantification of both human and mouse immune cells.
For each CD marker, we strive to provide antibodies tested in several applications to guarantee sensitivity and specificity. Please see example data below for WB, ICC, IHC, and flow cytometry with our recombinant anti-integrin alpha 5 antibody (ab150361). Find out more about recombinant antibodies.
ELISA PLATE READER : MICRO READ 1000 |
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1000 | Global Diagnostics B | 1 unit | 2628 EUR |
ELISA PLATE SHAKER : MICRO SHAKE 1200 |
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1200 | Global Diagnostics B | 1 unit | 916.8 EUR |
ELISA PLATE WASHER : MICRO WASH 1100 |
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1100 | Global Diagnostics B | 1 unit | 2403.6 EUR |
Dendritic Cells/B Cells Antibody |
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abx412775-02mg | Abbexa | 0.2 mg | 678 EUR |
Rat Pan B Cells antibody |
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10R-B124a | Fitzgerald | 50 ug | 910.8 EUR |
Human Cord Blood CD19+ B Cells |
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CBCD19-C2.5M | 101Bio | 2.5 million | 906 EUR |
Human Cord Blood CD19+ B Cells |
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CBCD19-C5M | 101Bio | 5 million | 1078.8 EUR |
Human Cord Blood CD19+ B Cells |
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CBCD19-F5M | 101Bio | 5 million | 1198.8 EUR |
Human B lymphocyte / GFP Stable Cells |
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SC060-G | GenTarget | 2 x 106 cell/ml x 1ml | 2670 EUR |
Human B lymphocyte / RFP Stable Cells |
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SC060-R | GenTarget | 2 x 106 cell/ml x 1ml | 2670 EUR |
Streptococcus Group B protein (inactivated cells) |
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30-AS86 | Fitzgerald | 1 ml | 236.4 EUR |
Human B lymphocyte (Luciferase / GFP) Stable Cells |
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SC060-LG | GenTarget | 2 x 106 cell/ml x 1ml | 3540 EUR |
Human B lymphocyte (Luciferase / RFP) Stable Cells |
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SC060-LR | GenTarget | 2 x 106 cell/ml x 1ml | 3540 EUR |
anti-IgM Heavy chain expressed on B cells |
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522-A-01mg | Virogen | 0,1 mg | 321 EUR |
anti-IgM Heavy chain expressed on B cells |
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522-A-1000ug | Virogen | 1000 ug | 1539 EUR |
anti-IgM Heavy chain expressed on B cells |
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522-A-100ugvial | Virogen | 100 ug/vial | 150 EUR |
5637 cells |
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C0002001 | Addexbio | One Frozen vial | 582 EUR |
FO cells |
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C0003017 | Addexbio | One Frozen vial | 582 EUR |
K1 cells |
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C0025003 | Addexbio | One Frozen vial | 792 EUR |
RD cells |
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C0035001 | Addexbio | One Frozen vial | 546 EUR |
A9 cells |
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P0011007 | Addexbio | One Frozen vial | 651.6 EUR |
BJ cells |
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P0020005 | Addexbio | One Frozen vial | 546 EUR |
ST cells |
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P0028002 | Addexbio | One Frozen vial | 651.6 EUR |
RS4;11 cells |
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C0003012 | Addexbio | One Frozen vial | 546 EUR |
REH cells |
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C0003031 | Addexbio | One Frozen vial | 582 EUR |
4T1 cells |
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C0006004 | Addexbio | One Frozen vial | 546 EUR |
T84 cells |
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C0009008 | Addexbio | One Frozen vial | 546 EUR |
RKO cells |
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C0009012 | Addexbio | One Frozen vial | 546 EUR |
H23 cells |
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C0016005 | Addexbio | One Frozen vial | 546 EUR |
H69 cells |
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C0016065 | Addexbio | One Frozen vial | 546 EUR |
H82 cells |
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C0016066 | Addexbio | One Frozen vial | 546 EUR |
A10.7 cells |
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C0018006 | Addexbio | One Frozen vial | 792 EUR |
C32 cells |
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C0020002 | Addexbio | One Frozen vial | 546 EUR |
N87 cells |
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C0023002 | Addexbio | One Frozen vial | 546 EUR |
AGS cells |
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C0023004 | Addexbio | One Frozen vial | 546 EUR |
R2C cells |
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C0028001 | Addexbio | One Frozen vial | 651.6 EUR |
Y79 cells |
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C0029001 | Addexbio | One Frozen vial | 546 EUR |
NB3 cells |
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C0033003 | Addexbio | One Frozen vial | 792 EUR |
STO cells |
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P0011006 | Addexbio | One Frozen vial | 546 EUR |
TM3 cells |
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T0028002 | Addexbio | One Frozen vial | 546 EUR |
MB49 cells |
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C0002004 | Addexbio | One Frozen vial | 932.4 EUR |
K562 cells |
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C0003004 | Addexbio | One Frozen vial | 546 EUR |
U937 cells |
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C0003005 | Addexbio | One Frozen vial | 546 EUR |
Raji cells |
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C0003009 | Addexbio | One Frozen vial | 546 EUR |
BJAB cells |
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C0003016 | Addexbio | One Frozen vial | 651.6 EUR |
Mino cells |
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C0003033 | Addexbio | One Frozen vial | 582 EUR |
B103 cells |
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C0005003 | Addexbio | One Frozen vial | 932.4 EUR |
T47D cells |
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C0006001 | Addexbio | One Frozen vial | 546 EUR |
HeLa cells |
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C0008001 | Addexbio | One Frozen vial | 546 EUR |
SiHa cells |
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C0008002 | Addexbio | One Frozen vial | 546 EUR |
C33A cells |
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C0008003 | Addexbio | One Frozen vial | 546 EUR |
LoVo cells |
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C0009011 | Addexbio | One Frozen vial | 546 EUR |
SW48 cells |
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C0009014 | Addexbio | One Frozen vial | 546 EUR |
H716 cells |
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C0009022 | Addexbio | One Frozen vial | 651.6 EUR |
OE33 cells |
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C0013003 | Addexbio | One Frozen vial | 792 EUR |
A549 cells |
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C0016002 | Addexbio | One Frozen vial | 546 EUR |
H460 cells |
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C0016003 | Addexbio | One Frozen vial | 546 EUR |
A427 cells |
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C0016004 | Addexbio | One Frozen vial | 546 EUR |
H520 cells |
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C0016010 | Addexbio | One Frozen vial | 546 EUR |
H226 cells |
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C0016011 | Addexbio | One Frozen vial | 546 EUR |
H358 cells |
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C0016016 | Addexbio | One Frozen vial | 546 EUR |
H441 cells |
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C0016019 | Addexbio | One Frozen vial | 546 EUR |
H596 cells |
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C0016020 | Addexbio | One Frozen vial | 546 EUR |
H526 cells |
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C0016052 | Addexbio | One Frozen vial | 546 EUR |
H446 cells |
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C0016054 | Addexbio | One Frozen vial | 651.6 EUR |
H187 cells |
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C0016061 | Addexbio | One Frozen vial | 546 EUR |
H209 cells |
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C0016063 | Addexbio | One Frozen vial | 546 EUR |
H847 cells |
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C0016067 | Addexbio | One Frozen vial | 651.6 EUR |
H660 cells |
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C0016068 | Addexbio | One Frozen vial | 834 EUR |
A673 cells |
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C0016074 | Addexbio | One Frozen vial | 546 EUR |
H524 cells |
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C0016077 | Addexbio | One Frozen vial | 651.6 EUR |
H211 cells |
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C0016078 | Addexbio | One Frozen vial | 651.6 EUR |
H146 cells |
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C0016080 | Addexbio | One Frozen vial | 546 EUR |
H889 cells |
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C0016081 | Addexbio | One Frozen vial | 651.6 EUR |
H128 cells |
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C0016083 | Addexbio | One Frozen vial | 651.6 EUR |
Vcap cells |
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C0019001 | Addexbio | One Frozen vial | 546 EUR |
8505C cells |
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C0025002 | Addexbio | One Frozen vial | 792 EUR |
MULTIPLEX KIT PCR MASTITIS PCR kit | |||
PCR-MPX218-48D | |||
MULTIPLEX KIT PCR MASTITIS PCR kit | |||
PCR-MPX218-96D | |||
PCR Mix | |||
L5051100 |