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Immobilization of Proteinase K for urine pretreatment to improve diagnostic accuracy of active tuberculosis

Immobilization of Proteinase K for urine pretreatment to improve diagnostic accuracy of active tuberculosis

September 25, 2021
BRD4 inhibition promotes TRAIL-induced apoptosis by suppressing the transcriptional activity of NF-κB in NSCLC

BRD4 inhibition promotes TRAIL-induced apoptosis by suppressing the transcriptional activity of NF-κB in NSCLC

October 5, 2021
Multifocal tuberculosis simulating multimetastatic colon cancer in an immunocompetent black African patient: a case report
DNA

Inhalable nanoparticles delivery targeting alveolar macrophages for the treatment of pulmonary tuberculosis

September 25, 2021
Super-enhancer-based identification of a BATF3/IL-2R-module reveals vulnerabilities in anaplastic large cell lymphoma
DNA

Super-enhancer-based identification of a BATF3/IL-2R-module reveals vulnerabilities in anaplastic large cell lymphoma

October 5, 2021
Crystal Structure of Mycobacterium tuberculosis Elongation Factor G1
DNA

Crystal Structure of Mycobacterium tuberculosis Elongation Factor G1

September 25, 2021
Isolation of Adult Human Astrocyte Populations from Fresh-frozen Cortex using Fluorescence-Activated Nuclei Sorting
DNA

Isolation of Adult Human Astrocyte Populations from Fresh-frozen Cortex using Fluorescence-Activated Nuclei Sorting

October 5, 2021
Mycobacterium tuberculosis Rv0927c Inhibits NF-κB Pathway by Downregulating the Phosphorylation Level of IκBα and Enhances Mycobacterial Survival
DNA

Mycobacterium tuberculosis Rv0927c Inhibits NF-κB Pathway by Downregulating the Phosphorylation Level of IκBα and Enhances...

September 25, 2021
Development of Human Toxo IgG ELISA Kit, and False-Positivity of Latex Agglutination Test for the Diagnosis of Toxoplasmosis
DNA

Development of Human Toxo IgG ELISA Kit, and False-Positivity of Latex Agglutination Test for the...

October 5, 2021
A Survey of Latent Tuberculosis Screening and Treatment Practices in a Tertiary Centre
DNA

A Survey of Latent Tuberculosis Screening and Treatment Practices in a Tertiary Centre

September 27, 2021
qpcr covid, SARS-CoV-2 UK, South African and Brazilian Variants in Karachi- Pakistan
DNA

SARS-CoV-2 UK, South African and Brazilian Variants in Karachi- Pakistan

November 16, 2021
CheXaid: deep learning assistance for physician diagnosis of tuberculosis using chest x-rays in patients with HIV
DNA

CheXaid: deep learning assistance for physician diagnosis of tuberculosis using chest x-rays in patients with...

September 27, 2021
Real-Time Spatial and Temporal Analysis of the Translocation of the Apoptosis-Inducing Factor in Cells
DNA

Real-Time Spatial and Temporal Analysis of the Translocation of the Apoptosis-Inducing Factor in Cells

November 16, 2021
12Page 1 of 2
  • Application of Metabolism Assay Kits in Drug Screening and Toxicology Studies
  • Implementing a Decentralized Quality Control Strategy for Abbott ID NOW™ Influenza Testing in Point-of-Care (POC) Settings
  • The Importance of PCR Qualitative Positive Controls in Diagnostic Assays: Ensuring Reliable Results
  • Optimizing Giemsa Stain PR80 for Accurate Cytogenetic Analysis in Cancer Diagnostics
  • Standardization and Optimization of oxLDL-R1 ELISA for Cardiovascular Disease Biomarker Detection
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POPULAR POSTS

Multifocal tuberculosis simulating multimetastatic colon cancer in an immunocompetent black African patient: a case report

Inhalable nanoparticles delivery targeting alveolar macrophages for the treatment of pulmonary tuberculosis

September 25, 2021
https://www.tbdb.org/

L-amino acid oxidase from snake venom: Biotransformation and induction of apoptosis in human colon cancer...

November 16, 2021
Diagnosis of intra-thoracic tuberculosis (ITTB) in children is difficult due to the paucibacillary nature of the disease, the challenge in collecting appropriate specimens, and the low sensitivity of smear microscopy and culture. Culture and Xpert MTB/RIF provide higher diagnostic yield in presumptive TB in adults than in children. Current study was designed to understand poor yield of diagnostic assays in children. Children with presumptive ITTB were subjected to gastric aspirates and induced sputum twice. Samples were tested by Ziehl-Neelsen stain, Xpert MTB/RIF-assay, and MGIT-960 culture. Subjects were grouped as Confirmed, Unconfirmed, and Unlikely TB, and classified as progressive primary disease (PPD, lung parenchymal lesion), and primary pulmonary complex (PPC, hilar lymphadenopathy) on chest X-ray. Of children with culture-positive TB 51/394 (12.9%), culture-negative TB 305 (77.4%), and unlikely TB 38 (9.6%), 9 (2.3%) were smear positive, while 95 (24.1%) were Xpert-MTB/RIF positive. Xpert-MTB/RIF detected 40/51 culture confirmed cases (sensitivity 78.4% and NPV 96.3%). Culture was positive in more children presenting as PPD (p < 0.04). In culture-negative TB group, Xpert positivity was seen in 31% of those with PPD and 11.9% in those with PPC (p < 0.001). Conclusion: Xpert-MTB/RIF improved diagnosis by 2-fold and increased detection of MDR-TB. Both liquid culture and Xpert-MTB/RIF gave higher yield in children with lung parenchymal lesions. Children with hilar lymphadenopathy without active lung parenchymal lesions had poor diagnostic yield even with sensitive nucleic acid amplification tests, due to paucibacillary/localized disease, suggesting possible utility of invasively collected samples in early diagnosis and treatment.

Diagnosis of intra-thoracic tuberculosis (ITTB) in children is difficult due to the paucibacillary nature of...

September 28, 2021

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