Purpose Information of latent tuberculosis an infection (LTBI) screening and remedy practices are missing in Eire, the place LTBI shouldn’t be programmatically surveyed or managed. The goal of this analysis was to explain present scientific apply when screening and treating sufferers for LTBI in a tertiary referral centre in Eire.
Strategies A 17-question survey referring to LTBI screening and administration practices with each open-ended questions and shut ended multiple-choice questions was created utilizing SurveyMonkey.
The survey goal pattern was healthcare staff within the tertiary centre who direct LTBI screening and remedy for sufferers vulnerable to TB illness of their respective departments. Outcomes The response fee to the survey was 45% (21/47).
Seventy-one % (15/21) of these surveyed responded to the query “What limitations exist to screening sufferers for latent TB in your scientific apply?”. Fifty-three % (8/15) mentioned that they discovered it tough to entry LTBI testing and 27% (4/15) cited accessing the interferon-gamma launch assay (IGRA) consequence as a barrier.
Forty-three % (9/21) responded that there was not a transparent referral pathway for sufferers that they want specialist enter on when diagnosing and managing sufferers with LTBI. Conclusion Entry to LTBI testing, LTBI check outcomes, TB specialist providers and using rifamycin-based regimens must be improved on this tertiary centre. Consideration must be given to creating a nationwide LTBI training programme for healthcare professionals and updating nationwide LTBI remedy tips.
Longitudinal Dynamics of a Blood Transcriptomic Signature of Tuberculosis
Targets We evaluated longitudinal kinetics of an 11-gene blood transcriptomic tuberculosis (TB) signature, RISK11, and results of TB preventative remedy (TPT) and respiratory organisms on RISK11 signature rating, in HIV-uninfected and HIV-infected people.
Strategies RISK11 was measured in a longitudinal examine of RISK11-guided TPT in HIV-uninfected adults, a cross-sectional respiratory organisms cohort or a longitudinal examine in folks dwelling with HIV (PLHIV). HIV-uninfected RISK11+ contributors have been randomised to TPT or no TPT; RISK11- contributors acquired no TPT.
PLHIV acquired standard-of-care ART and TPT. Within the cross-sectional respiratory organisms cohort, viruses and micro organism in nasopharyngeal and oropharyngeal swabs have been quantified by RT-qPCR. Measurements and Primary Outcomes RISK11+ standing was transient in a lot of the 128 HIV-negative contributors with longitudinal samples; >70% of RISK11+ contributors reverted to RISK11- by Three months, no matter TPT.
By comparability, reversion from a RISK11-positive state was much less widespread in 645 PLHIV (42.1%). Non-HIV viral and non-tuberculous bacterial organisms have been detected in 7.2% and 38.9% of the 1,000 respiratory organisms cohort contributors, respectively, and amongst these investigated for TB, 3.8% had prevalent illness.
Median RISK11 scores (%) have been increased in contributors with viral organisms alone (46.7%), viral and bacterial organisms (42.8%), or prevalent TB (85.7%), than these with bacterial organisms apart from TB (13.4%), or no organisms (14.2%). RISK11 couldn’t discriminate between prevalent TB and viral organisms.
Conclusions Optimistic RISK11 signature standing is usually transient, possibly resulting from intercurrent viral an infection, highlighting doubtlessly essential challenges for implementation of those biomarkers as new instruments for TB management. This text is open entry and distributed below the phrases of the Artistic Commons Attribution 4.zero Worldwide License.
Surgical administration of spinal tuberculosis with a two-stage posterior instrumentation with bridging: a case report
Tuberculosis (TB) impacts hundreds of thousands of individuals yearly. Spinal TB is a typical extrapulmonary manifestation of the illness. Spinal TB will be devastating and carries an unlucky final result.
Herein, we current an atypical spinal TB that was handled initially primarily based on intraoperative cultures with posterior decompression and instrumentation of T11-L3 with directed antibiotic remedy. Recurrence of the lesion and failure of instrumentation necessitated additional investigation and intervention 1 12 months later.
Utilizing a two-stage surgical process leaving the contaminated backbone to heal first with directed anti-TB medicines. The affected person was managed utilizing posterior instrumentation with bridging from T5 to the pelvis, spanning the destructed space and using a bridging approach with a number of rod constructs throughout the contaminated backbone. Right here, we current the advantage of utilizing the bridging approach to advertise bone therapeutic and obtain a strong fixation.
USE OF 15 MIRU-VNTR GENOTYPING FOR DISCRIMINATING M. TUBERCULOSIS CLINICAL ISOLATES FROM KAZAKHSTAN
Tuberculosis is likely one of the fundamental issues of drugs in Kazakhstan. Kazakhstan is on the record of 30 nations with excessive charges of multidrug resistant tuberculosis on the planet.
Purpose of this examine is to conduct genotyping by MIRU-VNTR technique to get preliminary knowledge on M. tuberculosis genotypes distributed among the many scientific isolates in Kazakhstan. 271 M. tuberculosis scientific isolates have been gathered from new circumstances of tuberculosis from completely different areas of Kazakhstan on this examine.
Genotyping was achieved utilizing 15 MIRU-VNTR (12 MIRU+Three ETR) loci. Obtained digital profiles of the scientific isolates have been analyzed utilizing the database on miru-vntrplus.org. Phylogenetic tree was constructed by UPGMA technique. 97 genotypes have been recognized, 70 (25.8%) of them have been distinctive and have been decided in a single isolate within the pattern assortment.
The remaining 201 (74.2%) isolates have been grouped into 27 clusters, that contained from 2 to 102 isolates. In response to genotyping outcomes M. tuberculosis Beijing household strains have been present in 65.3% circumstances. 121 out of 177 Beijing isolates (68.4%) have been drug-resistant. Prevalence of MDR-TB was detected amongst drug-resistant Beijing (58.7% – 71/121) and LAM household (50% – 10/20) isolates.
A Uncommon Case of Tuberculosis as a Explanation for Lytic Lesion of Talus With out Adjoining Bone Involvement in a 4-12 months-Outdated Baby
Further-pulmonary tuberculosis nonetheless stays an essential differential prognosis for power musculoskeletal illnesses in creating nations like India and will contain any a part of the physique with out attribute systemic options.
We’re presenting a uncommon case of a four-year-old feminine little one, who got here to our tertiary-care hospital with chief complaints of ache within the left foot together with a regularly rising swelling over the dorsum of the foot for the previous 5 months.
There was no historical past of trauma or constitutional signs. The serum inflammatory markers have been discovered raised, and X-ray and magnetic resonance imaging revealed an remoted lytic lesion within the talus bone. Debridement, in addition to curettage of the lesion, was achieved, each as a diagnostic and therapeutic process.
A caseous tacky materials was evacuated and despatched for microbiological and histopathological analysis, which revealed the presence of acid-fast bacilli and granulomatous lesion confirming the prognosis of tuberculosis.
The affected person was began with anti-tubercular chemotherapy, which continued for a complete length of 14 months, together with foot and ankle immobilization in a below-knee forged for 3 months.
After completion of remedy, there was full decision of the lytic lesion on x-ray, with full symptom aid, and a full vary of motion of the ankle was obtained.
In circumstances with longstanding ache and swelling of the foot, with or with out related systemic signs, tuberculosis must be thought of as a robust differential prognosis even in younger kids, particularly in creating nations.
 96-Well Protein Folding Plate |
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PFS096 |
ProFoldin |
kit |
EUR 347.84 |
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Description: This product includes one 96-well plate with 96 protein folding solutions, 0.5 ml in each well of the mother plate; 1.4 ml of Inclusion Body Solubilizer; 4 ml of Neutralizer. Each experiment uses 0.1 ml of the solutions from the mother plate. Each mother plate contains 0.5 ml of solutions in each well and can be used for multiple experiments of folding various proteins. |
 Monkey Mycobacterium Tuberculosis IgM ELISA kit, 96 tests, Quantitative |
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990-410-MTM |
Alpha Diagnostics |
1 Kit |
EUR 1000.8 |
) 96 well filter plate (960ul each well) |
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SD5006 |
Bio Basic |
12UNIT |
EUR 115.85 |
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 96 WELL CLEAR PCR PLATE FOR ABI |
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PCR-96-AB-C |
CORNING |
10/pk |
EUR 200.4 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
 Mycobacterium tuberculosis IgM ELISA Kit |
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DEIA383 |
Creative Diagnostics |
96T |
EUR 696 |
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Description: The Mycobacterium tuberculosis IgM antibody ELISA kit has been designed for the detection andthe quantitative determination of specific IgM antibodies against Mycobacterium tuberculosis in serum and plasma. |
 Human Anti-Mycobacterium Tuberculosis IgM ELISA kit, 96 tests, Quantitative |
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990-120-THM |
Alpha Diagnostics |
1 kit |
EUR 708 |
 1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY |
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P-DW-12-HC |
CORNING |
5/pk |
EUR 182.4 |
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Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen |
 Clear Coated 96 Well Plate, 1EA |
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C107-1EA |
Arbor Assays |
1EA |
EUR 226.8 |
) 96 well filter plate (no filter) |
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SD5006-EMPTY |
Bio Basic |
12UNIT |
EUR 104.89 |
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 INTELLI-PLATE 96-2 Shallow well |
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MAR-102-0001-20 |
MiTeGen |
120 PLATES |
EUR 990 |
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Description: INTELLI-PLATE 96-2 Shallow well; Â 2-well sitting drop, 120 plates |
 96 WELL SITTING DROP HIGH THROUGHPUT CRYSTALLOGRAPHY PLATE. |
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CP-AXYGEM-96-50 |
CORNING |
10/pk |
EUR 548.4 |
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Description: Crystallography Plates; Crystallography Plates - Axygen |
 96 WELL CLEAR PCR HALF SKIRT AMPLIFICATION PLATE |
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PCR-96-HS-C |
CORNING |
10/pk |
EUR 190.8 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
 Mycobacterium tuberculosis IgM ELISA Kit, Sensitive |
|
DEIA386 |
Creative Diagnostics |
96T |
EUR 696 |
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Description: The Mycobacterium tuberculosis IgM antibody ELISA kit has been designed for the detection andthe quantitative determination of specific IgM antibodies against Mycobacterium tuberculosis in serum and plasma. |
) 96 well DNA plate with membrane (960ul each well) |
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SD5007 |
Bio Basic |
12UNIT |
EUR 291.24 |
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) 96 well RNA plate with membrane (960ul each well) |
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SD5009 |
Bio Basic |
12UNIT |
EUR 313.16 |
|
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 Radius 96-Well Cell Migration Assay |
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CBA-126 |
Cell Biolabs |
96 assays |
EUR 686.4 |
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Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
Radius 96-Well Cell Migration Assay |
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CBA-126-5 |
Cell Biolabs |
5 x 96 assays |
EUR 2697.6 |
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Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
 CytoSelect 96-well Cell Transformation Assay |
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CBA-130 |
Cell Biolabs |
96 assays |
EUR 866.4 |
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Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader. |
CytoSelect 96-well Cell Transformation Assay |
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CBA-130-5 |
Cell Biolabs |
5 x 96 assays |
EUR 3463.2 |
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Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader. |
) CytoSelect 96-well Phagocytosis Assay (Zymosan) |
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CBA-224 |
Cell Biolabs |
96 assays |
EUR 866.4 |
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Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates. |
CytoSelect 96-well Phagocytosis Assay (Zymosan) |
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CBA-224-5 |
Cell Biolabs |
5 x 96 assays |
EUR 3525.6 |
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Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates. |
 OxiSelect 96-Well Comet Assay Kit |
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STA-355 |
Cell Biolabs |
96 assays |
EUR 512.4 |
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Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope. |
OxiSelect 96-Well Comet Assay Kit |
|
STA-355-5 |
Cell Biolabs |
5 x 96 assays |
EUR 1959.6 |
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Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope. |
 OxiSelect 96-Well Comet Assay Slide |
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STA-356 |
Cell Biolabs |
1 slide |
EUR 219.6 |
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Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy. |
OxiSelect 96-Well Comet Assay Slide |
|
STA-356-5 |
Cell Biolabs |
5 slides |
EUR 622.8 |
|
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy. |
 96-Well ROCK Activity Assay Kit |
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STA-416 |
Cell Biolabs |
96 assays |
EUR 1027.2 |
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Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1. |
96-Well ROCK Activity Assay Kit |
|
STA-416-5 |
Cell Biolabs |
5 x 96 assays |
EUR 4228.8 |
|
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1. |
 96 Well Breast Tumor Genomic DNA Plate |
|
D8235086-1 |
Biochain |
1 plate |
EUR 379.2 |
 96 Well Colon Tumor Genomic DNA Plate |
|
D8235090-1 |
Biochain |
1 plate |
EUR 379.2 |
 96 Well Lung Tumor Genomic DNA Plate |
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D8235152-1 |
Biochain |
1 plate |
EUR 379.2 |
 96 Well Stomach Tumor Genomic DNA Plate |
|
D8235248-1 |
Biochain |
1 plate |
EUR 379.2 |
 96-Well Plate PCR Products Purification Kit |
|
BS365 |
Bio Basic |
5XPlates, 480prep |
EUR 300.37 |
|
|
96-Well Plate PCR Products Purification Kit |
|
BS3652 |
Bio Basic |
2xPlates, 192prep |
EUR 186.19 |
|
|
 96-Well Plate DNA Cleanup Miniprep Kit |
|
BS369 |
Bio Basic |
5XPlates, 480prep |
EUR 300.37 |
|
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96-Well Plate DNA Cleanup Miniprep Kit |
|
BS3692 |
Bio Basic |
2xPlates, 192prep |
EUR 186.19 |
|
|
 96-Well Plate Plasmid DNA Miniprep Kit |
|
BS415 |
Bio Basic |
5XPlates, 480prep |
EUR 300.37 |
|
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96-Well Plate Plasmid DNA Miniprep Kit |
|
BS4152 |
Bio Basic |
2XPlates, 192prep |
EUR 186.19 |
|
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) Polystyrene-bottomed 96-well plate + lid (ELISPOT) |
|
CT365 |
U-CyTech |
5 |
EUR 93.6 |
 96-Well Plate Viral DNA Miniprep Kit |
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VT92032 |
Bio Basic |
2XPlates, 192prep |
EUR 495.86 |
|
|
 2 x Clear 96 well Plate, 2EA |
|
X003-2EA |
Arbor Assays |
2EA |
EUR 78 |
 5 x Clear 96 well Plate, 5EA |
|
X003-5EA |
Arbor Assays |
5EA |
EUR 96 |
2 x Clear 96 well Plate, 2EA |
|
X018-2EA |
Arbor Assays |
2EA |
EUR 78 |
 4 x Clear 96 Well Plate, 4EA |
|
X018-4EA |
Arbor Assays |
4EA |
EUR 104.4 |
 Black Half Area 96 Well Plate, 1EA |
|
X023-1EA |
Arbor Assays |
1EA |
EUR 96 |
 96 Well Impact MicroBatch Plate, 80/CSE |
|
M-673101 |
MiTeGen |
80 PLATES |
EUR 808 |
|
|
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Description: 96 Well Impact MicroBatch Plate, 80/CSE |
) SpotLiter? 96 well Plate Light Tracker (foot pedal & USB included) |
|
ACT-SL-96 |
ACTGene |
|
EUR 776.4 |
 ELISA test) TB (Mycobacterium Tuberculosis Antibody IgM) ELISA test |
|
39 |
Biobase |
96T/Box |
Ask for price |
|
|
|
Description: ELISA based test for quantitative detection of TB (Mycobacterium Tuberculosis Antibody IgM) |
 1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY PRE-STERILIZED |
|
P-DW-12-HC-S |
CORNING |
5/pk |
EUR 248.4 |
|
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen |
) CytoSelect 96-well Anoikis Assay (Colorimetric/Fluorometric) |
|
CBA-081 |
Cell Biolabs |
96 assays |
EUR 727.2 |
|
Description: Adhesion to the extraceullular matrix is essential for the survival and propagation of many adherent cells. Apoptosis resulting from the loss of adhesion to the ECM is known as anoikis. Anoikis is involved in the physiological processes of tissue renewal and cell homeostasis. Our CytoSelect Anoikis Assays allow you to quantify and monitor anoikis in cells using a precoated plate. Live cells can be viewed under a microscope and quantified on a plate reader by MTT (colorimetric) or Calcein AM (fluorometric); both reagents are included in the kit. Dead cells are detected with the red EthD-1 reagent, also included. |
 96-well Checkpoint Kinase Activity Assay Kit |
|
STA-414 |
Cell Biolabs |
96 assays |
EUR 908.4 |
|
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors. |
96-well Checkpoint Kinase Activity Assay Kit |
|
STA-414-5 |
Cell Biolabs |
5 x 96 assays |
EUR 3672 |
|
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors. |
 Block for 96 well plate, WSC-2620/30 |
|
4002659 |
Atto |
1unit |
EUR 458.4 |
|
Description: Bl ock for WSC-2620 / 2630 |
 96 Well Plate Blood Genomic DNA Miniprep Kit |
|
BT92031 |
Bio Basic |
2Plates, 192prep |
EUR 210.85 |
|
|
96 Well Plate Blood Genomic DNA Miniprep Kit |
|
BT92032 |
Bio Basic |
5Plates, 480prep |
EUR 389.9 |
|
|
 96 Well Plate RNA Cleanup and Concentration Kit |
|
BRC1251 |
Bio Basic |
2xPlates, 192prep |
EUR 300.37 |
|
|
 96-Well Plate Animal Genomic DNA Miniprep Kit |
|
BS437 |
Bio Basic |
5XPlates, 480prep |
EUR 435.58 |
|
|
96-Well Plate Animal Genomic DNA Miniprep Kit |
|
BS4372 |
Bio Basic |
2XPlates, 192prep |
EUR 234.6 |
|
|
 96 Well Plate Yeast Genomic DNA Miniprep Kit |
|
BS8357 |
Bio Basic |
2XPlates, 192prep |
EUR 234.6 |
|
|
 96-Well Plate Plant Genomic DNA Miniprep Kit |
|
BS8361 |
Bio Basic |
2XPlates, 192prep |
EUR 234.6 |
|
|
) PVDF membrane-bottomed 96-well plate + lid (ELISPOT) |
|
CT366 |
U-CyTech |
5 |
EUR 268.8 |
) 96-well Plasmid DNA Extraction Kit (1 plate) |
|
FAPWE-96001 |
Favorgen |
1 prep |
EUR 175.2 |
 96-Well Plate Bacteria Genomic DNA Miniprep Kit |
|
SK1292 |
Bio Basic |
2XPlates, 192prep |
EUR 210.85 |
|
|
96-Well Plate Bacteria Genomic DNA Miniprep Kit |
|
SK1295 |
Bio Basic |
5XPlates, 480prep |
EUR 389.9 |
|
|
Diagnostic and therapeutic curettage together with anti-tubercular chemotherapy may end up in a very good practical final result in such sufferers.
