Testing for SARS-CoV-2 in resource-poor settings stays a substantial problem. Gold normal nucleic acid checks are costly and depend upon availability of costly gear and extremely educated laboratory employees. Extra inexpensive and simpler speedy antigen checks are a horny various.
This examine assessed area performance of such a take a look at in western Kenya. We carried out a potential multi-facility area analysis examine of NowCheck COVID-19 Ag-RDT in comparison with gold normal PCR. Two pairs of oropharyngeal and nasopharyngeal swabs have been collected for comparative evaluation.
With 997 enrolled members the Ag-RDT had a sensitivity 71.5% (63.2-78.6) and specificity of 97.5% (96.2-98.5) at cycle threshold worth <40. Highest sensitivity of 87.7% (77.2-94.5) was noticed in samples with cycle threshold values ≤30. NowCheck COVID-19 Ag-RDT carried out properly at a number of healthcare services in an African area setting. Operational specificity and sensitivity have been near WHO-recommended thresholds.
The coronavirus illness 2019 (COVID-19) are outbreaking all around the world. To assist battle this illness, it’s obligatory to determine an efficient and speedy detection technique. The nucleocapsid (N) protein of Extreme Acute Respiratory syndrome Coronavirus 2 (SARS-CoV-2) is concerned in viral replication, meeting and immune regulation and performs an necessary function within the viral life cycle. Furthermore, the N protein additionally could possibly be a diagnostic issue and potential drag goal.
Subsequently, by synthesizing the N gene sequence of SARS-CoV-2, establishing the pET-28a (+)-N recombinant plasmid, we expressed the N protein in E.coli and obtained 15 mAbs towards SARS-CoV-2-N protein by the hybridomas and ascites, then an immunochromatographic take a look at strip technique detecting N antigen was established.
On this examine, we obtained 14 high-titer and high-specificity monoclonal antibodies, and the take a look at strips solely react with the SARS-CoV-2-N protein and no cross-reactivity with different coronavirus and likewise acknowledge the recombinant N protein of Delta (B.1.617.2) variant. These mAbs can be utilized for the early and speedy prognosis of SARS-CoV-2 an infection via serological antigen. This text is protected by copyright. All rights reserved.
PCR Mycoplasma Detection Kit | |
M034-Kit | TOKU-E |
MULTIPLEX KIT PCR MASTITIS PCR kit | |
PCR-MPX218-48D | Bioingentech |
MULTIPLEX KIT PCR MASTITIS PCR kit | |
PCR-MPX218-96D | Bioingentech |
MULTIPLEX KIT PCR Babesia & Theileria PCR kit | |
PCR-MPX401-48D | Bioingentech |
MULTIPLEX KIT PCR Babesia & Theileria PCR kit | |
PCR-MPX401-96D | Bioingentech |
Analysis of antigen-based speedy detection take a look at
Within the context of the Covid-19 pandemic and following the growing variety of suspicious Covid-19 instances in Madagascar, Malagasy laboratories are overflowed primarily as a result of lack of human resource and obtainable materials restriction. The event and validation of speedy and easy-to-perform diagnostic strategies are price of curiosity and excessive precedence. The goal of this potential examine was to guage the performances of a speedy immunochromatographic take a look at for the detection of SARS-CoV-2 antigen, compared to Reverse transcription polymerase chain response (RT-PCR).
The fluorescence immunochromatographic SARS-CoV-2 antigen take a look at StandardTM Q COVID-19 Ag Check (SD Biosensor Republic Korea) was evaluated in samples derived from sufferers who have been examined for illness classes. Diagnostic accuracy was decided compared to SARS-CoV-2 RT-PCR thought-about because the gold normal.
A complete of 200 samples have been included; 94 have been RT-PCR optimistic. Median sufferers’ age was 38.36 years, 63.5 % have been male. General sensitivity and specificity of the Commonplace TM Q COVID-19 Ag (SD Biosensor® Republic Korea) have been 62.66% and 100%, the sensitivity was considerably increased (100%) in samples with excessive viral hundreds (Ct<29).
This antigen-based immunofluorescence RDT could possibly be the potential to develop into an necessary device for the early prognosis of SARS-CoV-2 significantly in conditions with restricted entry to molecular strategies significantly in a rural areas of Madagascar.
PCR Tubes (50) | |||
PCR-50 | |||
MULTIPLEX KIT PCR MASTITIS PCR kit | |||
PCR-MPX218-48D | |||
MULTIPLEX KIT PCR MASTITIS PCR kit | |||
PCR-MPX218-96D |
Fast detection take a look at
To be able to help within the medical administration of sufferers and to assist an infection management, we examined using the SARS-CoV-2 point-of-care antigen take a look at (AgPOC) for unplanned hospitalization, coupled with a nucleic acid amplification take a look at (NAAT) utilizing specimens collected on the identical time upon arrival.
The goal of this examine was to evaluate the efficiency of the AgPOC on this particular use in comparison with NAAT for SARS-CoV-2 prognosis, in a context of low prevalence of an infection. For five months (between two peaks in France of the SARS-CoV-2 pandemic), all sufferers admitted who undertook the AgPOC/NAAT paired checks have been included within the examine. AgPOC performances have been decided contemplating medical standing and the delay of signs onset.
NAAT and AgPOC outcomes have been obtainable for 4,425 topics. AgPOC outcomes confirmed a homogeneous specificity (>97%) however a low sensitivity at 45.8%. Contemplating the nationwide pointers, sensitivity dropped to 32.5% in instances of symptomatic sufferers with signs older than 5 days or extra. This examine exhibits the poor efficiency of AgPOC for entry screening of sufferers in hospital.
SARS-CoV-2 Nucleocapsid Protein, Avi-His-tag |
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E80027 | EpiGentek |
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Recombinant SARS-CoV-2 Spike Glycoprotein(S) (D614G), Partial |
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E80028 | EpiGentek |
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SARS-CoV-2 Spike S1 RBD Protein, Avi-His-tag |
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E80024 | EpiGentek |
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SARS-CoV-2 Spike S1 RBD Protein, Mouse Fc-fusion |
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E80026 | EpiGentek |
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SARS-CoV-2 Spike S1 (16-685) Protein, Avi-His-tag |
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E80021 | EpiGentek |
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SARS-CoV-2 Spike S1 RBD (V367F) Protein, Avi-His-tag |
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E80023 | EpiGentek |
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SARS-CoV-2 Spike S1 (13-665) Protein, Fc Fusion, Avi-tag |
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E80020 | EpiGentek |
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SARS-CoV-2 Spike S1 (16-685) Protein, Fc Fusion, Avi-tag |
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E80022 | EpiGentek |
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SARS-CoV-2 Spike S1 RBD Protein, Human Fc-Fusion, Avi-Tag |
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E80025 | EpiGentek |
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3CL Protease (SARS-CoV-1 / SARS-CoV-2) Substrate |
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79952-2 | BPS Bioscience | 10 mg | 3460 EUR |
Spike (SARS-CoV-2) Lentivirus |
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78010-2 | BPS Bioscience | 500 µl x 2 | 2095 EUR |
3CL Protease (SARS-CoV-2) |
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100823-2 | BPS Bioscience | 500 µg_x000D_ | 3360 EUR |
PLPro, His-tag (SARS-CoV-2) |
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100735-2 | BPS Bioscience | 1 mg | 3000 EUR |
NSP10/NSP16 Complex (SARS-CoV-2) |
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100747-2 | BPS Bioscience | 1 mg | 2500 EUR |
NSP7, His-tag (SARS-CoV-2) |
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100829-2 | BPS Bioscience | 1 mg | 2600 EUR |
NSP8, His-tag (SARS-CoV-2) |
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100830-2 | BPS Bioscience | 1 mg | 2730 EUR |
Anti-Nucleocapsid Antibody (SARS-CoV-2 ) |
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100861-2 | BPS Bioscience | 100 µg | 420 EUR |
ORF9b, GST-Tag (SARS-CoV-2) |
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100962-2 | BPS Bioscience | 1 mg | 2720 EUR |
3CL Protease (SARS-CoV-2) Assay Kit |
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79955-2 | BPS Bioscience | 384 rxns. | 1265 EUR |
Spike S1, Fc fusion (SARS-CoV-2) |
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100688-2 | BPS Bioscience | 50 µg | 505 EUR |
Anti-Spike S1 Antibody (SARS-CoV-2) |
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100715-2 | BPS Bioscience | 100 µg | 440 EUR |
Spike S2, Fc-Tag (SARS-CoV-2) |
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100895-2 | BPS Bioscience | 500 µg_x000D_ | 1815 EUR |
SARS-CoV-2 Nucleocapsid Protein, Avi-His-tag |
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E80027-2 | EpiGentek | 100 ul | 4087.6 EUR |
3CL Protease, Untagged (SARS-CoV-2) Assay Kit |
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78042-2 | BPS Bioscience | 384 rxns. | 1210 EUR |
Spike (SARS-CoV-2) Pseudotyped Lentivirus (Luciferase Reporter) |
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79942-2 | BPS Bioscience | 500 µl x 2 | 4405 EUR |
Spike (SARS-CoV-2) Pseudotyped Lentivirus (eGFP Reporter) |
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79981-2 | BPS Bioscience | 500 µl x 2 | 5245 EUR |
Spike S1 RBD, His-tag (SARS-CoV-2) |
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100687-2 | BPS Bioscience | 100 µg | 520 EUR |
Spike S1 RBD, Fc fusion (SARS-CoV-2) |
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100699-2 | BPS Bioscience | 100 µg | 520 EUR |
3CL Protease (Mpro), MBP-tag (SARS-CoV-2) |
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100707-2 | BPS Bioscience | 1 mg | 2535 EUR |
Nucleocapsid Protein, Avi-His-tag (SARS-CoV-2) |
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100778-2 | BPS Bioscience | 1 mg | 2730 EUR |
Recombinant SARS-CoV-2 Spike Glycoprotein(S) (D614G), Partial |
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E80028-2 | EpiGentek | 100 ul | 860.2 EUR |
Spike (SARS-CoV-2, D614G) Pseudotyped Lentivirus (Luc Reporter) |
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78028-2 | BPS Bioscience | 500 µl x 2 | 4510 EUR |
Spike (SARS-CoV-2, D614G) Pseudotyped Lentivirus (eGFP Reporter) |
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78035-2 | BPS Bioscience | 500 µl x 2 | 5145 EUR |
Spike S1 RBD, Mouse Fc-fusion (SARS-CoV-2) |
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100684-2 | BPS Bioscience | 50 µg | 435 EUR |
Spike S1 RBD, Avi-His-tag (SARS-CoV-2) |
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100696-2 | BPS Bioscience | 1 mg | 3200 EUR |
Spike Trimer (S1+S2), His-tag (SARS-CoV-2) |
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100728-2 | BPS Bioscience | 1 mg | 2995 EUR |
Spike S1 RBD-Nucleocapsid Protein Chimera (SARS-CoV-2) |
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100938-2 | BPS Bioscience | 50 µg | 555 EUR |
3CL Protease (B.1.1.529, Omicron Variant), (SARS-CoV-2) |
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101328-2 | BPS Bioscience | 1 mg | 2750 EUR |
Spike (BA.2, Omicron Variant) (SARS-CoV-2) Pseudotyped Lentivirus (Luc Reporter) |
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78625-2 | BPS Bioscience | 500 µl x 2 | 4510 EUR |
Spike (BA.2, Omicron Variant) (SARS-CoV-2) Pseudotyped Lentivirus (eGFP Reporter) |
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78626-2 | BPS Bioscience | 500 µl x 2 | 4195 EUR |
3CL Protease (MERS-CoV) Substrate |
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78021-2 | BPS Bioscience | 1 mg | 1095 EUR |
SARS-CoV-2 Spike S1 RBD Protein, Avi-His-tag |
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E80024-2 | EpiGentek | 1 ml | 4995.1 EUR |
SARS-CoV-2 Spike S1 RBD Protein, Mouse Fc-fusion |
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E80026-2 | EpiGentek | 50 ul | 823.9 EUR |
Spike (SARS-CoV-2, UK Variant) Pseudotyped Lentivirus (Luc Reporter) |
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78112-2 | BPS Bioscience | 500 µl x 2 | 4405 EUR |
Spike (SARS-CoV-2) Pseudotyped VSV Delta G (Luciferase Reporter) |
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78637-2 | BPS Bioscience | 500 µl x 2 | 3995 EUR |
Spike(SARS-CoV-2) Pseudotyped Lentivirus (Luc-eGFP Dual Reporter) |
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79982-2 | BPS Bioscience | 500 µl x 2 | 8110 EUR |
Papain-like Protease (SARS-CoV-2) Assay Kit: Protease Activity |
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79995-2 | BPS Bioscience | 384 rxns. | 1240 EUR |
Spike S1 RBD, Fc-Fusion, Avi-Tag (SARS-CoV-2) |
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100698-2 | BPS Bioscience | 1 mg | 2500 EUR |
Spike S1 (16-685), Avi-His-tag (SARS-CoV-2) |
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100730-2 | BPS Bioscience | 1 mg | 2720 EUR |
Spike S1 RBD (V367F), Avi-His-tag (SARS-CoV-2) |
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100769-2 | BPS Bioscience | 1 mg | 2500 EUR |
Nucleocapsid Protein, Avi-His-tag, Biotin-Labeled (SARS-CoV-2) |
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100779-2 | BPS Bioscience | 50 µg | 435 EUR |
Spike S1 RBD (V483A), Avi-His-tag (SARS-CoV-2) |
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100846-2 | BPS Bioscience | 1 mg | 2600 EUR |
Spike S1 (B.1.351), Avi-His-Tag (SARS-CoV-2) |
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100992-2 | BPS Bioscience | 1 mg | 2850 EUR |
SARS-CoV-2 Spike S1 (16-685) Protein, Avi-His-tag |
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E80021-2 | EpiGentek | 1 ml | 4276.8 EUR |
SARS-CoV-2 Spike S1 RBD (V367F) Protein, Avi-His-tag |
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E80023-2 | EpiGentek | 1 ml | 3934.7 EUR |
Spike (B.1.351 Variant) (SARS-CoV-2) Pseudotyped Lentivirus (Luc Reporter) |
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78142-2 | BPS Bioscience | 500 µl x 2 | 4320 EUR |
Spike (K417T, E484K, N501Y) (SARS-CoV-2) Pseudotyped Lentivirus (Luc Reporter) |
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78143-2 | BPS Bioscience | 500 µl x 2 | 4195 EUR |
Spike (P.1 Variant) (SARS-CoV-2) Pseudotyped Lentivirus (Luc Reporter) |
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78144-2 | BPS Bioscience | 500 µl x 2 | 4195 EUR |
Spike (B.1.1.7 Variant) (SARS-CoV-2) Pseudotyped Lentivirus (eGFP Reporter) |
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78158-2 | BPS Bioscience | 500 µl x 2 | 4195 EUR |
Spike (P.1 Variant) (SARS-CoV-2) Pseudotyped Lentivirus (eGFP Reporter) |
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78159-2 | BPS Bioscience | 500 µl x 2 | 4195 EUR |
Spike (B.1.351 Variant) (SARS-CoV-2) Pseudotyped Lentivirus (eGFP Reporter) |
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78160-2 | BPS Bioscience | 500 µl x 2 | 4195 EUR |
3CL Protease (B.1.1.529, Omicron Variant) (SARS-CoV-2) Assay Kit |
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78350-2 | BPS Bioscience | 384 rxns. | 1315 EUR |
Spike (D614G) (SARS-CoV-2) Pseudotyped VSV Delta G (Luciferase Reporter) |
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78642-2 | BPS Bioscience | 500 µl x 2 | 3995 EUR |
Spike S1-Biotin (SARS-CoV-2): ACE2 TR-FRET Assay Kit |
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79949-2 | BPS Bioscience | 384 rxns. | 1265 EUR |
Spike S1 (13-665), Fc Fusion, Avi-tag (SARS-CoV-2) |
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100678-2 | BPS Bioscience | 1 mg | 3000 EUR |
Spike S1 RBD, Avi-His-tag, Biotin-labeled (SARS-CoV-2) |
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100697-2 | BPS Bioscience | 50 µg | 480 EUR |
Spike S1 (16-685), Fc Fusion, Avi-tag (SARS-CoV-2) |
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100719-2 | BPS Bioscience | 1 mg | 2720 EUR |
Spike S1 RBD, His-Avi-Tag, Biotin-Labeled (SARS-CoV-2) |
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100937-2 | BPS Bioscience | 50 µg | 435 EUR |
Spike RBD (B.1.1.7 Variant), Avi-His-Tag (SARS-CoV-2) |
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100977-2 | BPS Bioscience | 1 mg | 2850 EUR |
Spike RBD (B.1.351 Variant) Avi-His-Tag (SARS-CoV-2) |
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100978-2 | BPS Bioscience | 1 mg | 2850 EUR |
Nucleocapsid Protein (B.1.351 Variant), Avi-His-Tag (SARS-CoV-2) |
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100985-2 | BPS Bioscience | 1 mg | 2850 EUR |
Spike S1 (B.1.1.7 Variant), Avi-His-Tag (SARS-CoV-2) |
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101001-2 | BPS Bioscience | 1 mg | 2850 EUR |
Spike S1 (K417T, E484K, N501Y), Avi-His-Tag (SARS-CoV-2) |
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101081-2 | BPS Bioscience | 1 mg | 2850 EUR |
Spike S1 (B.1.617.2 Variant) Avi-His-Tag (SARS-CoV-2) |
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101151-2 | BPS Bioscience | 1 mg | 2995 EUR |
Spike (BA.2, Omicron Variant) (SARS-CoV-2) Pseudotyped VSV Delta G (Luciferase Reporter) |
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78635-2 | BPS Bioscience | 500 µl x 2 | 3995 EUR |
SARS-CoV-2 Spike S1 (13-665) Protein, Fc Fusion, Avi-tag |
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E80020-2 | EpiGentek | 1 ml | 4276.8 EUR |
SARS-CoV-2 Spike S1 (16-685) Protein, Fc Fusion, Avi-tag |
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E80022-2 | EpiGentek | 1 ml | 4276.8 EUR |
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