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Prediction of Multidrug-Resistant Tuberculosis Using Machine Learning Algorithms in SWAT, Pakistan

Prediction of Multidrug-Resistant Tuberculosis Using Machine Learning Algorithms in SWAT, Pakistan

September 28, 2021
Isolation of Adult Human Astrocyte Populations from Fresh-frozen Cortex using Fluorescence-Activated Nuclei Sorting

Isolation of Adult Human Astrocyte Populations from Fresh-frozen Cortex using Fluorescence-Activated Nuclei Sorting

October 5, 2021
basal salt, Understanding the electrochemistry of "water-in-salt" electrolytes: basal plane highly ordered pyrolytic graphite as a model system
Peptides

Understanding the electrochemistry of “water-in-salt” electrolytes: basal plane highly ordered pyrolytic graphite as a model...

November 16, 2021
Interplay and roles of oxidative stress, toll-like receptor 4 and Nrf2 in trichloroethene-mediated autoimmunity
Peptides

Interplay and roles of oxidative stress, toll-like receptor 4 and Nrf2 in trichloroethene-mediated autoimmunity

October 5, 2021
igg1k, A Retrospective Study to Assess the Efficacy, Safety and Drug Survival of Secukinumab in Plaque Psoriasis Patients in China
Peptides

A Retrospective Study to Assess the Efficacy, Safety and Drug Survival of Secukinumab in Plaque...

November 16, 2021
Tuberculosis presenting as metastatic lung cancer
Peptides

Tuberculosis presenting as metastatic lung cancer

September 27, 2021
A review of tuberculosis research in malaysia.
Peptides

A review of tuberculosis research in malaysia.

February 16, 2021
TCA cycle remodeling drives proinflammatory signaling in humans with pulmonary tuberculosis
Peptides

TCA cycle remodeling drives proinflammatory signaling in humans with pulmonary tuberculosis

September 25, 2021
Global trends of epidemiological research in livestock tuberculosis for the last four decades
Peptides

Global trends of epidemiological research in livestock tuberculosis for the last four decades

February 3, 2021
Advances in the research of an animal model of wound due to Mycobacterium tuberculosis infection
Peptides

Advances in the research of an animal model of wound due to Mycobacterium tuberculosis infection

February 3, 2021
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POPULAR POSTS

Multifocal tuberculosis simulating multimetastatic colon cancer in an immunocompetent black African patient: a case report

Inhalable nanoparticles delivery targeting alveolar macrophages for the treatment of pulmonary tuberculosis

September 25, 2021
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L-amino acid oxidase from snake venom: Biotransformation and induction of apoptosis in human colon cancer...

November 16, 2021
Diagnosis of intra-thoracic tuberculosis (ITTB) in children is difficult due to the paucibacillary nature of the disease, the challenge in collecting appropriate specimens, and the low sensitivity of smear microscopy and culture. Culture and Xpert MTB/RIF provide higher diagnostic yield in presumptive TB in adults than in children. Current study was designed to understand poor yield of diagnostic assays in children. Children with presumptive ITTB were subjected to gastric aspirates and induced sputum twice. Samples were tested by Ziehl-Neelsen stain, Xpert MTB/RIF-assay, and MGIT-960 culture. Subjects were grouped as Confirmed, Unconfirmed, and Unlikely TB, and classified as progressive primary disease (PPD, lung parenchymal lesion), and primary pulmonary complex (PPC, hilar lymphadenopathy) on chest X-ray. Of children with culture-positive TB 51/394 (12.9%), culture-negative TB 305 (77.4%), and unlikely TB 38 (9.6%), 9 (2.3%) were smear positive, while 95 (24.1%) were Xpert-MTB/RIF positive. Xpert-MTB/RIF detected 40/51 culture confirmed cases (sensitivity 78.4% and NPV 96.3%). Culture was positive in more children presenting as PPD (p < 0.04). In culture-negative TB group, Xpert positivity was seen in 31% of those with PPD and 11.9% in those with PPC (p < 0.001). Conclusion: Xpert-MTB/RIF improved diagnosis by 2-fold and increased detection of MDR-TB. Both liquid culture and Xpert-MTB/RIF gave higher yield in children with lung parenchymal lesions. Children with hilar lymphadenopathy without active lung parenchymal lesions had poor diagnostic yield even with sensitive nucleic acid amplification tests, due to paucibacillary/localized disease, suggesting possible utility of invasively collected samples in early diagnosis and treatment.

Diagnosis of intra-thoracic tuberculosis (ITTB) in children is difficult due to the paucibacillary nature of...

September 28, 2021

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