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Culture Cells

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CD4 T cell help prevents CD8 T cell exhaustion and promotes control of Mycobacterium tuberculosis infection

CD4 T cell help prevents CD8 T cell exhaustion and promotes control of Mycobacterium tuberculosis infection

September 28, 2021
basal salt, Understanding the electrochemistry of "water-in-salt" electrolytes: basal plane highly ordered pyrolytic graphite as a model system

Understanding the electrochemistry of “water-in-salt” electrolytes: basal plane highly ordered pyrolytic graphite as a model system

November 16, 2021
Tuberculosis presenting as metastatic lung cancer
Culture Cells

Tuberculosis presenting as metastatic lung cancer

September 27, 2021
qpcr covid, SARS-CoV-2 UK, South African and Brazilian Variants in Karachi- Pakistan
Culture Cells

SARS-CoV-2 UK, South African and Brazilian Variants in Karachi- Pakistan

November 16, 2021
A review of tuberculosis research in malaysia.
Culture Cells

A review of tuberculosis research in malaysia.

February 16, 2021
Diagnosis of intra-thoracic tuberculosis (ITTB) in children is difficult due to the paucibacillary nature of the disease, the challenge in collecting appropriate specimens, and the low sensitivity of smear microscopy and culture. Culture and Xpert MTB/RIF provide higher diagnostic yield in presumptive TB in adults than in children. Current study was designed to understand poor yield of diagnostic assays in children. Children with presumptive ITTB were subjected to gastric aspirates and induced sputum twice. Samples were tested by Ziehl-Neelsen stain, Xpert MTB/RIF-assay, and MGIT-960 culture. Subjects were grouped as Confirmed, Unconfirmed, and Unlikely TB, and classified as progressive primary disease (PPD, lung parenchymal lesion), and primary pulmonary complex (PPC, hilar lymphadenopathy) on chest X-ray. Of children with culture-positive TB 51/394 (12.9%), culture-negative TB 305 (77.4%), and unlikely TB 38 (9.6%), 9 (2.3%) were smear positive, while 95 (24.1%) were Xpert-MTB/RIF positive. Xpert-MTB/RIF detected 40/51 culture confirmed cases (sensitivity 78.4% and NPV 96.3%). Culture was positive in more children presenting as PPD (p < 0.04). In culture-negative TB group, Xpert positivity was seen in 31% of those with PPD and 11.9% in those with PPC (p < 0.001). Conclusion: Xpert-MTB/RIF improved diagnosis by 2-fold and increased detection of MDR-TB. Both liquid culture and Xpert-MTB/RIF gave higher yield in children with lung parenchymal lesions. Children with hilar lymphadenopathy without active lung parenchymal lesions had poor diagnostic yield even with sensitive nucleic acid amplification tests, due to paucibacillary/localized disease, suggesting possible utility of invasively collected samples in early diagnosis and treatment.
Culture Cells

Diagnosis of intra-thoracic tuberculosis (ITTB) in children is difficult due to the paucibacillary nature of...

September 28, 2021
Real-Time Spatial and Temporal Analysis of the Translocation of the Apoptosis-Inducing Factor in Cells
Culture Cells

Real-Time Spatial and Temporal Analysis of the Translocation of the Apoptosis-Inducing Factor in Cells

November 16, 2021
TCA cycle remodeling drives proinflammatory signaling in humans with pulmonary tuberculosis
Culture Cells

TCA cycle remodeling drives proinflammatory signaling in humans with pulmonary tuberculosis

September 25, 2021
Prediction of Multidrug-Resistant Tuberculosis Using Machine Learning Algorithms in SWAT, Pakistan
Culture Cells

Prediction of Multidrug-Resistant Tuberculosis Using Machine Learning Algorithms in SWAT, Pakistan

September 28, 2021
neuroscience antibodies, Nociceptor neurons promote IgE class switch in B cells
Culture Cells

Nociceptor neurons promote IgE class switch in B cells

November 16, 2021
Distinct blood transcriptomic signature of treatment in latent tuberculosis infected individuals at risk of developing active disease
Culture Cells

Distinct blood transcriptomic signature of treatment in latent tuberculosis infected individuals at risk of developing...

September 25, 2021
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  • Application of Metabolism Assay Kits in Drug Screening and Toxicology Studies
  • Implementing a Decentralized Quality Control Strategy for Abbott ID NOW™ Influenza Testing in Point-of-Care (POC) Settings
  • The Importance of PCR Qualitative Positive Controls in Diagnostic Assays: Ensuring Reliable Results
  • Optimizing Giemsa Stain PR80 for Accurate Cytogenetic Analysis in Cancer Diagnostics
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